文章摘要
邵一鸣,曾毅,陈筝,赵全壁,赵尚德,马瑛,贾曼红,杨文乔,段一娟,段松.从云南艾滋病病毒(HIV)感染者分离HIV[J].中华流行病学杂志,1991,12(3):129-135
从云南艾滋病病毒(HIV)感染者分离HIV
Isolation of Human Immunodeficiency Virus(HIV) in Epidemic Area of HIV infection in Yunnan Province
收稿日期:1990-10-01  出版日期:2021-06-07
DOI:
中文关键词: 人免疫缺损病毒  病毒分离
英文关键词: Human immunedeficiency virus  Virus isolation
基金项目:
作者单位
邵一鸣 中国预防医学科学院病毒学研究所 
曾毅 中国预防医学科学院病毒学研究所 
陈筝 中国预防医学科学院病毒学研究所 
赵全壁 中国预防医学科学院病毒学研究所 
赵尚德 云南省卫生防疫站 
马瑛 云南省卫生防疫站 
贾曼红 云南省卫生防疫站 
杨文乔 云南省瑞丽县卫生防疫站 
段一娟 云南省瑞丽县卫生防疫站 
段松 云南省德宏州卫生防疫站 
摘要点击次数: 1022
全文下载次数: 279
中文摘要:
      从云南人免疫缺损病毒(HIV)感染流行区感染者采血,用外周血单个核细胞(PMCs)共培养方法分离HIV,以逆转录酶测定法(RT)为检测终点。总共从24名无症状HIV抗体阳性者和一名持续性全身性淋巴腺病病人分离到10株HIV。经HIV1p 24 Elisa和HIV1 Pol和Gag基因引物多聚酶链反应(PCR)证实并鉴定所分离株均为HIV1型。与文献报道从同类人群所分离的毒株生物学特性相似,这些毒株仅在PMCs缓慢生长,不致细胞病变。目前正在改变培养条件以提高病毒滴度并对扩增的基因进行分析以寻找我国云南HIV流行株与世界其他地区HIV流行株的差异。
英文摘要:
      Blood were collected from HIV infected persons in epidemic area of HIV infection in Yunnan province for isolation of HIV.The coculture method was used for cultivating the virus and reverse transcriptase assay (RT) was the main method for detection of HIV. Of 25 seropositive, 24 asymptomatic and one PGL, 10 showed positive RT activity (>5 000 cpm/ml and with a steadily increase, some to more than 40 000 cpm/ml). The results were confirmed by the detection of HIV1 p24 Ag (ELISA)and HIV1 POL and GAG gene sequence (PCR)). In accordance with the reports from other labs, the viruses isolated from these group of persons infect only PMCs, grew slowly with gradual increase of RT activity and caused no CPE. Efforts are making, at present, to rise the virus titer with better culture system. The amplified gene sequence of the isolates are under investigating.
查看全文   Html全文     查看/发表评论  下载PDF阅读器
关闭