| 廖利民,石炳毅,梁春泉,庄玉辉,沈荣森.PCR技术检测尿中人巨细胞病毒的方法学研究[J].中华流行病学杂志,1995,16(2):111-113 |
| PCR技术检测尿中人巨细胞病毒的方法学研究 |
| Detection of Human Cytomegalovirus in Urine by Polymerase Chain Reaction |
| 收稿日期:1994-05-16 出版日期:2021-05-20 |
| DOI： |
| 中文关键词: 巨细胞病毒 肾移植 聚合酶链反应 |
| 英文关键词: HCMV Renal transplantation PCR |
| 基金项目: |
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| 中文摘要: |
| 应用HCMV早、晚期两对DNA引物,建立了聚合酶链反应(PCR)检测尿中人巨细胞病毒(HCMV)的方法。肾移植术后HCMV感染的临床尿样检测结果表明,该方法具有快速、特异性强、灵敏度高等优点,与血清HCMV-IgM检测结果符合率为83.3%。使用早、晚期基因组的双引物分别对临床尿样进行PCR扩增,减少了由于自然点突变所致的假阴性,且引物与HCMV同属其它病毒及组织DNA间无交叉反应;以HCMV组织培养液作模板,灵敏度可达625fg。本方法为诊断肾移植受者HCMV感染提供了新的手段,适于临床推广。 |
| 英文摘要: |
| Polymerase chain reaction (PCR) amplification was set up with double primer pairs of major immediateearly and late gene in order to detect human cytomegalovirus (HCMV) from urine. This method was applied to the detection of HCMV in clinical samples of urine from renal transplant recipients. The result indicated that the primers did not cross react with other members of the herpes family of virus and human genomic DNA; that the HCMV AD169 tissue culture mixture used and the dilutions to estimate the sensitivity of PCR relative to tissue culture, 2.5μl of a 10-3 dilution of the culture (625fg HCMV DNA) assayed were detected by direct gel analysis; that 25 of 30 samples of urine from the renal transplant recipients (serum HCMV IgM positive) were positive. It is conclude that PCR amplification is a valuable tool for diagnoses of HCMV infection in renal transplant recipients. |
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