文章摘要
杨世明,杨枨林,安仁鸣,解华.消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察[J].中华流行病学杂志,1995,16(3):154-156
消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察
Preliminary Observation on the Destructive Effect of Chemical Disinfectants in serum Markers of Hepatitis B and C
收稿日期:1994-01-16  出版日期:2021-05-22
DOI:
中文关键词: HBV  Anti-HCV  ELISA
英文关键词: HBV  Anti-HCV  ELISA
基金项目:
作者单位
杨世明 解放军第四军医大学唐都医院血库 710038 陕西省西安市 
杨枨林 解放军第四军医大学唐都医院血库 710038 陕西省西安市 
安仁鸣 解放军第四军医大学唐都医院血库 710038 陕西省西安市 
解华 解放军第四军医大学唐都医院血库 710038 陕西省西安市 
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中文摘要:
      笔者采用氯仿、40%甲醛溶液、3%双氧水及75%乙醇与HBsAg、HBeAg和抗-HCV阳性血清作用5、15、30及60分钟后,用ELISA法检测是否转为阴性,以了解消毒剂对上述阳性血清的破坏效果。结果:氯仿对HBsAg和抗-HCV阳性血清破坏效果好;40%甲醛溶液对HBsAg、HBeAg及抗-HCV阳性血清均破坏;3%双氧水对HBeAg有破坏作用,而75%乙醇对HBsAg、HBeAg及抗-HCV阳性血清作用上述时间后,检测时仍呈阳性反应。笔者只是初步观察,有关消毒剂的浓度、效果及作用机理有待迸一步观察和探讨。
英文摘要:
      Chloroform, 40% formaldehyde, 3% hydrogen peroxide solution and 75% alcohol were used to act on hepatitis B surface antigen (HBeAg), hepatitis B e antigen (HBaAg) and anti-hepatitis C virus antibody (Anti-HCV) positive serum for 5, 15, 30 and over 60 minutes. They were examined by enzyme-linked immunosorbent assay and observed for the effect.
The destructive effect of chloroform on HBsAg and anti-HCV positive derum was marked. It was discovered that 40% formaldehyde solution destroyed HBsAg, HBeAg, and anti-HCV positive serm, while 75% alcohol also showed destructive effect on HBsAg, HBeAg and anti-HCV positive serum.
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