| 李新武,李秋丽,李涛,孙守宏,袁洽劻.一种戊二醛复方消毒剂杀灭微生物效果观察[J].中华流行病学杂志,1996,17(5):292-295 |
| 一种戊二醛复方消毒剂杀灭微生物效果观察 |
| Experimental Observation on Microbicidal Activity of a Complex Glutaraldehyde Disinfectant |
| 收稿日期:1996-04-20 出版日期:2021-05-15 |
| DOI： |
| 中文关键词: 戊二醛 消毒 灭菌 |
| 英文关键词: Glutaraldehyde Disinfection Sterilization |
| 基金项目: |
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| 摘要点击次数: 1040 |
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| 中文摘要: |
| 本文观察了一种戊二醛复方消毒剂对微生物的杀灭效果,结果表明,在试验温度为20℃,试验用菌悬液中加入10%小牛血清的条件下,该消毒剂2.00%的稀释液作用2分钟可杀灭99.99%的金黄色葡萄球菌,1.00%的稀释液作用2分钟可杀灭99.99%的大肠杆菌,原液作用35分钟可破坏HBsAg的抗原性;在加热50℃条件下,原液作用35分钟,50.00%的稀释液作用45分钟可100%杀灭枯草杆菌黑色变种芽胞。在对该消毒剂的有效抑菌、杀菌和根据能量试验确定的应用浓度比较时发现,杀菌浓度比抑菌浓度高4倍,应用浓度比杀菌浓度高5倍,此结果提示,在消毒试验中,选择适当的中和剂十分重要;消毒剂的实际应用浓度应根据能量试验的结果来确定。 |
| 英文摘要: |
| The microbicidal activity of a complex glutaraldehyde disinfectant was observed. The result showed that when the temperature of the test was 20℃ and 10% calf serum were added to the bacterial suspensions 2% diluted solution of the disinfectant for a two-minute contact could kill 99.99% of Staphylococcus aureus, and 1% diluted solution for two minutes could kill 99.99% of E.coli. The undiluted disinfectant with five-minute contact time could destroy the antigenicity of HBsAg. The undiluted disinfectant with thirty-five minute contact and 50% diluted solution with forty-five minute contact time could completely kill the spores of Bacillus subtilis var. niger under 50℃. It was discovered that the germicidal concentration of the disinfectant was four times as high as the inhibition concentration and the used concentration determinal with capacity test was four times higher than as germicidal concentration when the three concentrations were compared. This result indicated that selection of suitable inactivator was very important. The practical concentration of disinfectant should be decided under the basis of the result from capacity test. |
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