文章摘要
孙英杰,Li Jin,葛力.1995~1997年中国部分地区麻疹病毒流行株基因型分析[J].中华流行病学杂志,1998,19(6):330-333
1995~1997年中国部分地区麻疹病毒流行株基因型分析
Geneotic Analysis of Wild Type Measles Viruses Circulating in China During 1995-1997
收稿日期:1998-07-20  出版日期:2014-09-18
DOI:
中文关键词: 麻疹病毒  RT-PCR  序列分析
英文关键词: Measles virus  RT-PCR Sequence  analysis
基金项目:
作者单位
孙英杰 辽宁省卫生防疫站,沈阳 110005 
Li Jin Enteric Respiratory VirusLaboratory Central Public Health Laboratory 
葛力 辽宁省大连市卫生防疫站 
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中文摘要:
      本文报道以反转录二步多聚酶链反应方法(RT-PCR)直接测定麻疹病人唾液、尿液标本中的麻疹病毒(MV)血凝基因(H)、核衣壳基因(N)。以RT-PCR产物纯化后进行MV450ntH、282ntN基因核苷酸片段测序分析,测序结果一致表明,1995~1997年期间中国部分地区MV流行株存在两种基因型。其中一种基因型只发现1株,与EDw54株相同,另一种基因型占流行株的绝大多数(称新基因型),与其它国家以前报道的MV流行株基因型的N基因最大差异为15.4%与EDw54、疫苗株相差8.8%;H基因最大差异是10.8%。
英文摘要:
      To investigate the molecular epidemiology of measles virus (MV) in China, sequence analysis of MV was performed based on a 282-nt region of the nucleoprotein (N) gene and a 450-nt region of heamaglutinin (H) gene by direct sequencing of RT-PCR amplicons obtained from clinical specimens. Results revealed that one MV strain belonged to ED-like genotype and remainder strains were clustered into a new genotype which was distinct from Chinese vaccine strains and MV strains circulating in other countries, as previously described representing a new genotype. There was up to 15.4% divergence in the nt-282 N gene and 10.8% divergence in the nt-450 H gene between the new genotype and other genotypes.
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