| 陈杰,刘伟,NancyL,Young,ShambaviSubbarao,梁富雄,李荣健,梁绍伶,TimothyD,Mastro,SurangaSaguanwongse,SuthonWongsheree.广西HIV-1首次流行的分子流行病学分析[J].中华流行病学杂志,1999,20(2):74-76 |
| 广西HIV-1首次流行的分子流行病学分析 |
| Molecular-epidemiological analysis of HIV-1 initial prevalence in Guangxi,China |
| 收稿日期:1998-12-14 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: |
| 英文关键词: HIV-1 Genetic subtype Serotyping DNA sequence |
| 基金项目: |
| 作者 | 单位 | | 陈杰 | 广西壮族自治区卫生防疫站, 南宁 530021 | | 刘伟 | 广西壮族自治区卫生防疫站, 南宁 530021 | | NancyL | 广西壮族自治区卫生防疫站, 南宁 530021 | | Young | 广西壮族自治区卫生防疫站, 南宁 530021 | | ShambaviSubbarao | 广西壮族自治区卫生防疫站, 南宁 530021 | | 梁富雄 | 广西壮族自治区卫生防疫站, 南宁 530021 | | 李荣健 | 广西壮族自治区卫生防疫站, 南宁 530021 | | 梁绍伶 | 广西壮族自治区卫生防疫站, 南宁 530021 | | TimothyD | 广西壮族自治区卫生防疫站, 南宁 530021 | | Mastro | 广西壮族自治区卫生防疫站, 南宁 530021 | | SurangaSaguanwongse | 广西壮族自治区卫生防疫站, 南宁 530021 | | SuthonWongsheree | 广西壮族自治区卫生防疫站, 南宁 530021 |
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| 中文摘要: |
| 目的广西自1996年4月起在静脉吸毒者和卖血者中发现HIV感染者,为了解其传染来源和判断其流行趋势,对广西流行的HIV进行分子流行病学分析。方法 选取HIV抗体阳性血清标本44份,分别采用多肽酶免疫法（PEIA）和经逆转录PCR扩增作cDNA序列分析,分别确定其HIV-1基因亚型并加以比较。结果结果表明广西存在4种HIV-1M组基因亚型,即B‘（泰国B亚型）、C、D、E亚型。在静脉吸毒人群和性混乱者中存在HIV-1E亚型流行和C亚型感染者；而在卖血者中发现HIV-1B’和D亚型感染。结论 HIV-1D亚型感染和E亚型流行已在国内出现,E亚型病毒已由东南亚传入流行并将在我国南部形成新的流行区域。提出血清学分型方法可作为HIV-1基因亚型分析的筛选技术推广应用。 |
| 英文摘要: |
| Objective In April 1996,HIV-1 infection was first found among both commercial blood donors and IDUs in Guangxi.In order to identify the source of HIV-1 transmission and analyze the trend of HIV-1 epidemic,the study was carried out.Methods HIV-1 genetic subtypes were determined by peptide enzyme immunoassays (PEIA),RT-PCR and DNA sequencing.Results Four subtypes of Group M HIV-1 were found in Guangxi:subt ype B(Thai B),C,D and E.Subtype E and C (1 person) were circulating among IDUs and hetrosexual,while subtype B and D (1 person) were among commercial blood donors. Subtype B infections were discovered among a group of commercial blood donors and one of them was infected by subtype D. Conclusion It is the first report of subtype D infection and subtype E that were identified in China.This observation indicated that subtype E HIV-1 was spread into China from Southeast Asia,and a new epidemic region with subtype E HIV-1 would emerge in southern China. Serotyping might be a useful screening method for HIV-1 molecular epidemiological analysis |
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