| 程伯鲲,崔树玉,温宪芹,姜桂香,李连青,刘巧突,赵斌秀,叶长芸,徐建国.我国腹泻病患者和家畜家禽粪便标本中携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌的调查[J].中华流行病学杂志,2000,21(2):130-133 |
| 我国腹泻病患者和家畜家禽粪便标本中携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌的调查 |
| Detection of Escherichia coli strains harboring pathogenicity island of Yersinia enterolitica in diarrheal patients and animals in China |
| 收稿日期:1999-12-20 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 致泻性大肠杆菌 耶尔森氏菌 毒力岛 腹泻 |
| 英文关键词: Diarrhea g enic E.coli Yersinia enterocolitica Pathogenicity island Diarrhea |
| 基金项目:国家自然科学基金杰出青年基金资助项目(39625001);国家重点基础研究发展规划项目(G1999054101) |
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| 中文摘要: |
| 目的 了解携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌在我国腹泻病患者粪便标本、动物粪便标本及部分食品标本中的存在情况和所致腹泻病的临床特征. 方法 使用菌落原位杂交、DNA打点杂交和PCR扩增等方法. 结果 在各地送检的从腹泻病患者粪便标本中分离到的、用常规方法不能鉴定的大肠杆菌中, 均检出带有小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌菌株, 检出率为27. 05%(436/16 12). 从市售食品标本中分离的大肠杆菌中, 携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌的检出率为10. 23%(9/88). 从家畜家禽粪便中分离的大肠杆菌中, 携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌检出率为5. 71%(16/280). 携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌所致腹泻的典型临床表现为食欲不振、腹痛、寒战乏力、正常体温或低热、每日腹泻多在6次以上、多为粘液便. 结论 携带小肠结肠炎耶尔森氏菌HPI毒力岛的大肠杆菌在我国分布广泛, 检出率高于其他几类致泻性大肠杆菌, 对我国人民的健康是一个潜在的威胁. |
| 英文摘要: |
| Objective To investigate the prevalence of Escherichia coil strains harboring high pathogenicity island of Yersinia enterocolitica in diarrhea patients, animals, food samples and resulted clinical symptoms. Methods Colony hybridization、DNA dot hybridization and polymerase chain reaction (PCR) were used. Results HPI-harboring E. coli were found in the strains isolated in various provinces of China. The isolation rates were 27. 05% (436/1 612) in the strains isolated from diarrhea patients, 10. 23 % (9/88) in the strains isolated from food samples and 5. 71% (16/280) in the strains isolated from feces of cattle and pigs. Typical clinical symptoms of diarrhea caused by HPI-harboring E, coli were inappetency, abdominal pain, shiver and vapidity. Patient's temperature was usually normal or low. Over six episodes of diarrhea was frequently observed, of which most were unformed stools with mucous. Conclusion E. coli strains harboring high-pathogenicity-island of Yersinia species were frequently isolated from diarrhea patients, animals and food samples as well, which might be one of the major causes of diarrhea in China. |
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