文章摘要
孙红妹,刘哲伟,张霆.反义寡聚核苷酸抗柯萨奇病毒感染的研究[J].中华流行病学杂志,2000,21(4):295-297
反义寡聚核苷酸抗柯萨奇病毒感染的研究
Antisense oligonucleotides resistance to coxsackievirus B3 infection in HeLa cells
收稿日期:2000-03-17  出版日期:2014-09-16
DOI:
中文关键词: 反义寡聚核苷酸;柯萨奇病毒;HeLa细胞
英文关键词: Antisense oligodeoxynucleotides(ODNs);Coxsakievirus;HeLa cells
基金项目:
作者单位
孙红妹 首都儿科研究所, 北京市感染与免疫中心实验室, 100020 
刘哲伟 首都儿科研究所, 北京市感染与免疫中心实验室, 100020 
张霆 首都儿科研究所, 北京市感染与免疫中心实验室, 100020 
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中文摘要:
      目的 针对柯萨奇病毒-3型(CVB3)基因组重要功能区设计系列反义寡聚核苷酸序列,通过实验观察特异性反义核酸对病毒感染细胞的抑制作用。方法 通过病毒空斑形成试验及致细胞病变作用,了解反义核酸抑制及阻断CVB3病毒感染细胞的能力。结果 实验所设计的反义核酸中SCB1对感染HeLa细胞的CVB3病毒活性抑制作用较强,当病毒感染量为1MOI时,病毒活性抑制率高达90%;SCB4和SCB2均为75%;SCB6有50%抑制作用;而SCB3和SCB5以及非特异性对照序列对CVB3病毒基因基本没有任何作用。结论 通过以上实验确定SCB1、SCB2、SCB4等较好的抗病毒反义核酸序列,为进一步研究反义核酸抗病毒作用提供了重要的实验基础。
英文摘要:
      Objective Antisense oligodeoxynucleotides (ODNs) hold great promise as therapeutic agents for the treatment of many human diseases. Oligonucleotides specifically hybrid with a selected sequence of RNA or DNA and inhibit the flow of genetic information DNA (or RNA) to proteins. In accordance with the present study, several oligonucleotide sequences that targeted CVB3 RNA coding Vp4, Vp2, Vp1, cleavage enzyme(P2A) or untranslational region were designed for observation. Methods Antiviral activities of these oligonucleotides were evaluated by plaque reduction assay and cytopathic effect (CPE). Cells were also treated with random oligonocleotides as a specificity control. Results Original ODNs, displaying sequence-specific inhibition ranging from 50% to 90% were identified. Three ODNs (at 5 microM concentration), which targeting at the pyrimidine - rich tract (SCB1), structural protein Vp1 (SCB4) and initiation of translation (SCB2) were shown to exhibit inhibition of viral infection 75% to 90% at 1 MOI. There was only low antiviral activity in SCB5 and SCB3, and random oligonucleotide were not found having antiviral activity. Oligonucleotides in this study were not toxic at 5 microM concentration. Conclusion A better antiviral antisense oligonucleotides as SCB1 and SCB4、SCB2, could provide candidate antisense oligonucleotides for the treatment of CVB3 infection.
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