| 黄芳,俞东征,海荣,蔡虹.随机扩增多态性DNA技术用于鼠疫耶尔森氏菌基因分型的研究[J].中华流行病学杂志,2000,21(6):424-426 |
| 随机扩增多态性DNA技术用于鼠疫耶尔森氏菌基因分型的研究 |
| Study on the application of randomamplified polymorphic DNA in Yersinia pestis genotyping |
| 收稿日期:2000-06-12 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 鼠疫耶尔森氏菌 随机扩增多态性DNA技术 |
| 英文关键词: Yersinia pestis Random amplified polymorphic DNA |
| 基金项目: |
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| 中文摘要: |
| 目的 对来自全国不同疫源地、不同生态型的 103株鼠疫耶尔森氏菌进行基因分型研究。方法 用随机扩增多态性DNA技术 (RAPD)。结果 将鼠疫耶尔森氏菌分成两种基因型别 :全国大部分菌株为RAPD-1型,青海省境内的大部分菌株为RAPD-2型。结论 不同生态型的鼠疫耶尔森氏菌基因结构存在差异,为鼠疫耶尔森氏菌的基础研究和防治提供依据 |
| 英文摘要: |
| Objective To study the type of 103 Y.pestis strains that isolated from different plague foci and different ecolog ical types.Method Random amplified polymo rphic DNA (RAPD)was used.Results Y.pestis w ere divided into two RAPD types :most strains that isolated from elsewhere in the country belo nged to RAPD-1 ty pe, while mo st strains that iso lated from Qinghai province were RAPD-2 type.Conclusion The genome structures of different eco ty pe Y.pestis were different providing thefoundation of fur ther research and prevention of plague. |
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