|Homogeneity study on the Streptococcus suis isolated from human and swine
|中文关键词: 猪链球菌 菌体脂肪酸 基因
|英文关键词: Streptococcus suis Thallifatty acid profile Gene
| 目的 进一步鉴定菌种 ；评价人源株与猪源株猪链球菌的同源性。方法 对分离自猪无菌部位、患者血液和脑脊液的 7株猪链球菌Ⅱ型和标准猪链球菌Ⅱ型,用菌体脂肪酸分析和随机引物基因扩增技术进行菌种的表现型和基因型分类。对其结果进行聚类分析和主成分分析。结果 随机引物基因扩增技术分析提示,被检的 7株猪链球菌Ⅱ型与标准株一致,均为猪链球菌Ⅱ型 ；人源株与猪源株同源 ；分离自病人血液和脑脊液的菌株同源。这些结果在菌体脂肪酸分析中得到了进一步的确认。即基因型与表现型结果一致。结论 被检的 7株猪链球菌Ⅱ型与标准株均同源
| Objective To identify S.suis and to evaluate the homogeneity of isolates of S.suis from human and swine.Methods Culture, mo rphology, API biochemical tests and serum coagulate tests were used.All the 7 strains o f streptococcus were confirmed to be S.suis sero ty pe 2.Two strains and 1 strain were isolated from blood and cerebrospinal fluid (CSF)of patients, respectively, w hile the other 4 strains were obtained from the infected swine or their co rpses.Seven strains of S.suis serotype 2 andreference strain SS2 w ere analy zed by randomly amplified polymorphic DNA analy sis with six primers and Rep PCR with four primers and thalli fatty acid profile analysis.Cluster and principal compound analysis of results were performed with RAPD, Phy lip and Treeview software.Results By analysis on RAPD patterns, a close relationship among 7 strains of S.suis sero type 2 and reference strain SS2 was discovered. Human-born strains and swine-bo rn strains ex hibit similar RAPD patterns.There was a same clonal relationship betw een strains o riginated from the blood and CFS of the patients.These results were confirmed by thalli fatty acid profile to have genotypic and pheno ty pic identity. Conclusion Homogeneity exists among the 7 strains and reference strain SS2.