| 汪萱怡,马景臣,张勇,张英林,张玉伟,韩常全,邢占春,陈吉朝,赵守军,徐志一.不同剂量甲型肝炎减毒活疫苗免疫原性与免疫持久性观察[J].中华流行病学杂志,2001,22(2):111-113 |
| 不同剂量甲型肝炎减毒活疫苗免疫原性与免疫持久性观察 |
| Immunogenicity and long- term persistence of ant- i HAV in groups with different attenuated and inactived hepatitis A vaccine dosage |
| 收稿日期:2000-10-19 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 甲型肝炎 疫苗,减毒 免疫原性 抗体 |
| 英文关键词: Hepatitis A Vaccine, attenuated Immunogenicity Antibody |
| 基金项目:国家/ 九五0科技攻关项目( 96 -906 -03 -01) |
|
| 摘要点击次数: 3072 |
| 全文下载次数: 889 |
| 中文摘要: |
| 目的观察不同剂量的甲型肝炎(甲肝)减毒活疫苗免疫原性与免疫持久性。方法对河北省正定县4个村166名6～8岁事先经血清检测抗HAV阴性的易感儿童，按村分成4组，分别给予0.5、1、2及2剂经60℃3h灭活处理的甲肝减毒活疫苗(滴度为106.52TCID50)。并于接种后3、12、24、36及48个月采集观察对象血清，采用AbbottIMxmEIA试剂检测血清抗HAV。并对接种1剂、2剂活疫苗组免后4年部分血清检测中和抗体。结果免后3个月抗体阳转率、抗体几何平均滴度(GMT)同疫苗滴度呈剂量效应关系。免疫后各组抗体水平于免疫后3个月达高峰后逐年下降，12个月内下降较明显，12个月后下降缓慢。免疫后4年接种2剂活疫苗组抗体阳性率与GMT均高于接种0.5剂及1剂组。接种1、2剂活疫苗组部分经AbbottIMxmEIA试剂检测抗HAV阴性的血清检测中和抗体，其阳性率为60%及83%。结论甲型肝炎减毒活疫苗的免疫原性与免疫持久性同疫苗的滴度有关，滴度为106.83TCID50的减毒活疫苗具有较理想的免疫原性及免疫持久性。减毒活疫苗接种后近期内不必予以加强免疫。 |
| 英文摘要: |
| Objective T his study was carried out to assess the long -term persistence of antibodies against hepatitis A in different vaccine dosage groups. Methods One hundred and sixty -six susceptibles aged form 6 to 8 years old were selected in Zhengding county, and were divided into 4 different dose groups. Each group received 10 6. 17 TCID 50, 10 6. 52 TCID 50, 10 6. 83 TCID 50 of attenuated hepatitis A vaccine or 10 6. 83 TCID 50 of the same vaccine but treated with 60e for 3 hours. All subjects were bled at month 3,12, 24, 36 and 48. All serum specimens were tested for antibodies to HAV using Abbott-IMx mEIA reagent. Part of the tested negative serum specimens were retested by neutralization assay for 10 6. 52 TCID 50 and 10 6. 83 TCID 50 doseage groups at 4 years after vaccination. Results The seroconversion rate and GMT 3 months after vaccination in each dosage group showed a significant dose -response relationship. Antibody levels reached the peak at month 3 in each group but declined dramatically within one year and the declineslowed down thereafter. The serocoversion rate and GMT in 10 6. 83 TCID 50 dose group 4 years after vaccination was significant higher than those of other dosage groups. Positive rates of neutralizing antibodies in B and C dosage group were 60. 0% and 83. 3% respectively. Conclusion T he immunogenicity and long -term persistence were related to the attenuated hepatitis A vaccine titer. The vaccine, whose titer was 10 6. 83 T CID 50, had good immunogenicty and long -term persistence. Booster dose was not needed withinshort time after initial vaccination. |
|
查看全文
Html全文
查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
