| 姚智慧,董关木,张佳珂,俞永新,刘学成,刘文雪,张灵麟,阎侗有.汉坦病毒四川分离株S85-46 的遗传学特征[J].中华流行病学杂志,2001,22(6):449-451 |
| 汉坦病毒四川分离株S85-46 的遗传学特征 |
| Analysis on the genetics of Hantavirus S85-46 isolated from Sichuan province |
| 收稿日期:2001-02-02 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 汉坦病毒 遗传学特征 序列测定 |
| 英文关键词: Hantavirus Genetics analysis Sequencing |
| 基金项目: |
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| 中文摘要: |
| 目的 了解汉坦病毒四川分离株S85-46 株分子流行病学特征。方法 将特异性引物从S85-46 病毒感染细胞PCR 扩增的产物克隆于T 载体，正确的克隆纯化后测序， 应用DNASTAR 软件比较分析。结果 S85-46 株M 片段与H TN 型毒株同源性为84.1 %～ 99.7%，而与SEO 型毒株同源率仅为70.4%～ 70.9%， 表明S8-46 毒株属HTN 型。核苷酸同源性比较显示， S85-46 与韩国分
离的76-118 株高度同源， 而与国内一些HTN 型病毒差异较大， 而且同一地区的毒株也可以差异很大。S 片段全基因序列同源性比较也支持以上的观点。结论 不同地区汉坦病毒的流行株基因序列可以高度同源。 |
| 英文摘要: |
| Objective To understand the molecular and epidemic characteristics of Hantavirus S85-46 isolated from Sichuan province.Methods RT-PCR products w ere amplified from S85-46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR softw are.Results The homolog y of M segment of S85-46 strain and HTN type strain varied from 84.1%-99.7 %, compared to 70.4 %-70.9% w ith SEO strains.This result indicated that S85-46 strain belonged to hantavirus.The nucleo tide sequence analyses indicated that S85-46 strain, LR1 strain and 76-118 strain were hig hly homologous.The two latter strains were isolated from Shaanxi province of China and South Korea respectively, which were far from Sichuan province of China.The nucleotide sequence analyses of S segment was similar to Msegment.Conclusion The epidemic strains isolated from different regio ns may have been highly
homologous |
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