文章摘要
曲梅,阚飙,祁国明,刘延清,高守一.中国O139群霍乱弧菌核糖体基因多态性的研究[J].中华流行病学杂志,2002,23(3):203-205
中国O139群霍乱弧菌核糖体基因多态性的研究
A study on genetic polymorphism of rRNA gene pattern of Vibrio cholerae O139 in China
收稿日期:2001-09-11  出版日期:2014-09-17
DOI:
中文关键词: 霍乱弧菌  O139群  核糖体基因分型
英文关键词: Vibrio cholerae  serogroup O139  Ribotyping
基金项目:国家自然科学基金资助项目(39870031)
作者单位E-mail
曲梅 中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室 北京 102206  
阚飙 中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室 北京 102206 kanb@btamail.net.cn 
祁国明 中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室 北京 102206  
刘延清 中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室 北京 102206  
高守一 中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室 北京 102206  
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中文摘要:
      目的 分析国内O139群霍乱弧菌分离株的遗传多态性. 方法 以16srRNA和23srRNA核糖体基因作探针, 对内切酶消化的菌株染色体DNA进行Bouthern杂交, 分析杂交图谱. 结果 选择的122株O139群霍乱弧菌得到10种核糖体基因型. 9株ctxAB、zot和RS均为阴性的无毒菌株, 分属于4种独特的核糖体基因型. 结论 O139群霍乱弧菌在遗传分化及克隆群的地区分布上存在多态性, 提示国内O139霍乱流行的复杂性.
英文摘要:
      Objective To investigated the genetic polymorphism of the isolated strains using ribotyping method. Methods One handred twenty two strains of V. cholerae O139 isolated from different areas of China from 1993 to 1999 were selected and characterized with ribotyping, including 16s rDNA and 23s rDNA probes. Results One handred twenty two strains were differentiated into 10 different ribotypes (RT1 RT10) on the basis of rRNA gene probes hybridization (with BglⅠ digestion), which consisted of 7 9 bands between 12 and 1. 5 kb in size. RT1 and RT3, as two predominant ribotypes, comprised most number of the strains which spread to the extensive range. Nine strains, which are negative to ctxAB, zot and RS individually, belong to 4 special ribotypes. The dendrogram revealing genetic relationship among different clones of V. cholerae O139 showed that the clones belonging to RT1 and RT2 had genetic similarity on high degree, although they were isolated from different regions. The two predominant ribotypes (RT1 and RT3) were distant in genetic relationship. Conclusion Results showed the clonal diversity and the wide area distribution of V. cholerae O139 strains in China, suggesting the multiple origins of O139 epidemics.
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