| 俞东征,海荣,董兴齐,李敏,夏连续,史献明,魏建春,崔百忠,王鹏,孙连芝,张志凯,胡源,张恩民.中国鼠疫菌种资源遗传特征分析[J].中华流行病学杂志,2003,24(11):1005-1009 |
| 中国鼠疫菌种资源遗传特征分析 |
| GenetiCanalysis of Yersiniapestis strains isolated in China |
| 收稿日期:2003-06-18 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 鼠疫菌 资源 遗传特征 |
| 英文关键词: Yersiniapestis Resource Genetic characteristic |
| 基金项目: |
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| 中文摘要: |
| 目的建立《中国甲类传染病菌种资源库》,选择适当的指标,以反映中国鼠疫菌种的遗传背景。方法使用传统的和分子生物学方法,测定中国鼠疫菌种代表菌株的遗传特征。并对遗传特征的分布及其意义进行分析。结果利用6项指标,将中国的鼠疫菌划分成15种遗传型,每一型占据一片相对独立的而又自相延续的地理空间。分析遗传型之间的亲缘关系,并根据菌株间的差异,追溯鼠疫耶尔森菌作为一个生物种形成后的进化过程。结论中国各鼠疫自然疫源地存在着不同特征的鼠疫菌株,但为达到对菌株认同的目标,还需要确定更多的遗传指征 |
| 英文摘要: |
| Objective The strainsof Yersiniapestis isolated in differentperiod and differenTnatural foci in China were analyzed. Methods Traditional and molecular biological Methods were used. Rhamnose fermentantion, rRNA gene copy number, nitrite reduction, and the glycerol fernentation were importanTchararcters for typing, and pulse field gel electrophoresis( PFGE)and random amplified polymorphic DNA ( RAPD)profile could reflecTthe genetic distance between the strains.Results The strains could be dividedinto 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.Conclusion The characters of strains were described;the genetic relationship of defferenTtypes, their evolution, and the forming and shifTof plague natural foci were analyzed. |
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