文章摘要
汤芳,刘玮,张泮河,赵秋敏,詹琳,左曙青,吴晓明,杨红,方立群,魏茂提,王宏伟,曹务春.SARS患者粪便、痰标本病毒RNA阳性检出率与病程变化的关系[J].中华流行病学杂志,2004,25(2):120-123
SARS患者粪便、痰标本病毒RNA阳性检出率与病程变化的关系
Study on the correlation between positive rates of SARS RNA in clinical confirmed SARS patients and the appearance of RNA in relation to the development of the disease
收稿日期:2003-09-29  出版日期:2014-09-15
DOI:
中文关键词: 严重急性呼吸综合征  冠状病毒  逆转录-聚合酶链反应
英文关键词: Sever acute respiratory syndrome  SARS associated coronavirus  Reverse transcription-polymerase chain reaction
基金项目:国家重点发展计划“863”计划资助项目(2003AA208406)
作者单位
汤芳 军事医学科学院微生物流行病研究所, 北京 100071 
刘玮 军事医学科学院微生物流行病研究所, 北京 100071 
张泮河 军事医学科学院微生物流行病研究所, 北京 100071 
赵秋敏 军事医学科学院微生物流行病研究所, 北京 100071 
詹琳 军事医学科学院微生物流行病研究所, 北京 100071 
左曙青 军事医学科学院微生物流行病研究所, 北京 100071 
吴晓明 军事医学科学院微生物流行病研究所, 北京 100071 
杨红 军事医学科学院微生物流行病研究所, 北京 100071 
方立群 军事医学科学院微生物流行病研究所, 北京 100071 
魏茂提 军事医学科学院微生物流行病研究所, 北京 100071 
王宏伟 军事医学科学院微生物流行病研究所, 北京 100071 
曹务春 军事医学科学院微生物流行病研究所, 北京 100071 
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中文摘要:
      目的 探讨严重急性呼吸综合征(SARS)患者粪便、痰标本中病毒RNA阳性检出率与病程变化的关系。方法 采取一步法逆转录聚合酶链反应(RT-PCR)对不同病程的临床标本进行SARS冠状病毒(SARS-associated coronavirus,SARS-CoV)RNA的扩增和序列测定,并采用趋势X~2检验探讨标本阳性检出率与不同发病时间的关系。结果 被检样品中扩增出的特异性片段,经测序验证为SARS-CoV序列(同源性100%)。在检测的临床标本中,粪便标本阳性检出率最高(21.55%)。趋势X~2检验显示SARS患者粪便和痰标本中病毒阳性检出率均随着发病时间的增长而下降,X~2=12 55和16.408,P=0.0004和P=0.000 05。结论 一步法RT-PCR可以有效检测SARS患者临床标本存在的SARS-CoV;SARS患者粪便、痰标本RT-PCR阳性率随着发病时间的增长而下降。
英文摘要:
      Objectives To study the correlation between positive rates of RNA in clinical confirmed severe acute respiratory syndrome (SARS) patients and its appearance in relation to the development of the disease in order to provide scientific basis for early diagnosis, effective prevention and treatment of the disease. Methods One-step reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the SARS RNA in the clinical specimens from different courses of the disease. The representative amplicons were then sequenced. Chi-square for trend test was performed to study the correlation between positive rates of RT-PCR and at different periods after the onset of the disease. Results The fragments amplified from the sputum specimens of SARS patients were shown to share 100% homology with the published SARS- associated coronavirus. Of the different clinical specimens, positive rate in the stools appeared to be the highest (21. 55%). Chi-square for trend test revealed that the positive rates of stools and sputa of SARS patients decreased with the development of the disease (X~2 for trend = 12. 55 and 16. 408, P = 0. 0004 and P = 0. 000 05 respectively). Conclusion One-step RT-PCR proved to be an effective method for the detection of SARS-associated coronavirus from clinical specimens. Data as indicated that the positive rates o SARS coronavirus were decreasing in SARS patients along with the disease progession.
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