败酱草抗病毒有效部位体外抑制呼吸道合胞病毒作用研究

李珊珊; 李洪源; 朴英爱; 刘电力; 田文静; 董艳梅

文章摘要

李珊珊,李洪源,朴英爱,刘电力,田文静,董艳梅.败酱草抗病毒有效部位体外抑制呼吸道合胞病毒作用研究[J].中华流行病学杂志,2004,25(2):150-153

败酱草抗病毒有效部位体外抑制呼吸道合胞病毒作用研究

The anti-respiratory syncytial virus effect of an active compound (AP3) from a Chinese medicinal herb—Herba patriniae in vitro

收稿日期:2003-01-24 出版日期:2014-09-15

DOI：

中文关键词: 败酱草呼吸道合胞病毒抗病毒作用有效成分

英文关键词: Herba potriniae Respiratory syncytial virus Antiviral effect Active compounds

基金项目:国家自然科学基金(3017ll37)

作者	单位
李珊珊	哈尔滨医科大学公共卫生学院 150001
李洪源	哈尔滨医科大学公共卫生学院 150001
朴英爱	黑龙江省药品检验所
刘电力	哈尔滨医科大学公共卫生学院 150001
田文静	哈尔滨医科大学公共卫生学院 150001
董艳梅	哈尔滨医科大学公共卫生学院 150001

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中文摘要:

目的了解败酱草抗病毒有效部位（AP3）体外抑制呼吸道合胞病毒的作用。方法采用中药煎煮法、乙醇提取法和大孔吸附树脂层析法分离得到抗病毒AP3。采用细胞病变抑制试验,以病毒唑为阳性对照药,观察不同浓度AP3在HeLa细胞中对呼吸道合胞病毒的抑制作用,计算药物半数有效浓度(EC₅₀)和药物治疗指数（TI）。结果 AP3半数中毒浓度(TC₅₀)为11.45mg/ml；抑制呼吸道合胞病毒的EC₅₀为0.0986 mg/ml,TI为116.12；AP3对呼吸道合胞病毒的抑制作用存在量效反应关系；AP3于感染后0、2、4 h加药,有抑制呼吸道合胞病毒活性。结论败酱草抗病毒AP3在HeLa细胞中对呼吸道合胞病毒有明显的抑制作用。

英文摘要:

Objective To study the effect on anti--respiratory srncytial virus of an active compound (AP3) from a Chinese medicinal herb——Herba patriniae in vitro. Methods Active component of herba patriniae (AP3) was extracted and its anti--respiratory syncytial virus(RSV) effect was tested. A water soluble substance (AP3) was isolated from a Chinese herb Herba patriniae, by hot water extraction, ethol precipitation and gel permeation column chromatography. The cytotoxicity of AP3 was tested by adding it to HeLa cells directly. Its effect against RSV was estimated by CPEI assay while ribavirin was used as positive control. Results Chemical test showed that the nature of substance AP3 was polysaccharide. The median cytotoxic concentration (TC_(50)) of AP3 was 11. 45 mg/ml by morphological observation and the median effective concentration (50% effetive concentration, EC_(50)) of it against replication of the long strain of RSV in HeLa cells was 0. 0986 mg/ml. The Therapeutic index (TI= TC_(50)/EC_(50)) of AP3 was 116. 12, much higher than the TI of herba patriniae (AP1) (TI = 59. 26) and ribavirin (TI = 53. 45). Moreover, AP3 gave a dose-dependent response in inhibiting RSV. In the assay, the effect of AP3 against RSV growth was also tested. In addition, the effect of AP3 on virus growth, AP3 inhibited replication of RSV in HeLa cells, when added at 0 h, 2 h, 4 h after virus infection, were also tested. Conclusion This study suggested that the AP3 exerted an obvious inhibitory effect to RSV in HeLa cell culture. This study furnished a reliable evidence for development of a new antiviral drug.

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