SARS-CoV特异性抗体产生规律的初步研究

貌盼勇; 朱雷; 王佑春; 侯俊; 胡燕; 沈宏辉; 辛绍杰; 李保森; 毛远丽; 庄辉

文章摘要

貌盼勇,朱雷,王佑春,侯俊,胡燕,沈宏辉,辛绍杰,李保森,毛远丽,庄辉.SARS-CoV特异性抗体产生规律的初步研究[J].中华流行病学杂志,2004,25(10):856-858

SARS-CoV特异性抗体产生规律的初步研究

Study on the response of specific antibodies against SARS-CoV in patients infected with SARS

收稿日期:2003-11-05 出版日期:2014-09-18

DOI：

中文关键词: 严重急性呼吸综合征|病毒|特异性抗体

英文关键词: Severe acute respiratory syndrome|Virus|Specific antibody

基金项目:

作者	单位	E-mail
貌盼勇	100039 北京,解放军第三○二医院传染病研究所
朱雷	100039 北京,解放军第三○二医院传染病研究所
王佑春	中国药品生物制品检定所细胞室
侯俊	100039 北京,解放军第三○二医院传染病研究所
胡燕	100039 北京,解放军第三○二医院传染病研究所
沈宏辉	100039 北京,解放军第三○二医院传染病研究所
辛绍杰	100039 北京,解放军第三○二医院传染病研究所
李保森	100039 北京,解放军第三○二医院传染病研究所
毛远丽	100039 北京,解放军第三○二医院传染病研究所
庄辉	北京大学医学部病原微生物学系	zhuang.hui@hotmail.com

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中文摘要:

目的研究严重急性呼吸综合征(SARS)患者感染后体内病毒特异性抗体产生规律。方法收集临床确诊为SARS患者的血清和非SARS人群血清标本,用IgM捕获法、间接法和抗原夹心法三种不同方法检测抗SARS病毒特异性IgM、IgG和总抗体。结果检测146份临床诊断为SARS的患者不同发病时间血清标本,三种抗体阳性率分别为61.64%、53.43%和69.86%;SARS病毒特异性IgM、IgG抗体的最早检出时间分别在发病第7天和第12天,特异性IgM抗体最短在发病后42天消失。三种方法检测70份甲型肝炎患者血清时,均有2份非特异阳性反应,检测127份其他病种血清均阴性,1例密切接触SARS患者的医务人员SARS特异性IgG抗体和总抗体均阳性,三种检测方法均不受类风湿因子影响。结论与其他病毒感染相比,SARS病毒感染者的特异性IgM抗体检出时间较晚,且持续时间较短;三种检测方法均有较好的特异性和敏感性,可用于SARS的流行病学调查和临床诊断的确认和补充,但不适用于SARS的早期诊断。

英文摘要:

Objective To study the response of specific antibodies against severe acute respiratory syndrome( SARS)一CoV in patients infected with SARS. Methods IgM-capture, indirect and antigen- sandwiched enzyme linked immunosorbent assay (ELISA) were used to detect the SARSCoV specific IgM, IgG and total antibodies in sera of clinical SARS patients or non-SARS individuals. Results The positive rates of IgM, IgG and total antibodies to SARS-CoV in 146 sera of SARS patients collected in different phases of the disease were 61.64 % . 53 . 43 % and 69 . 86，respectively. The earliest detectable days after onset of the disease for IgM and IgG to SRAS-CoV were 7 and 12 days, respectively. The specific IgM disappeared as early as 42 days after the onset of SARS. Of 70 sera from hepatitis A patients. 2 showed false positive Results, while 127 sera from other patients were all negative, detected by the 3 Methods. Serum from one medical worker who had been close contact to SARS patients was positive for anti-SARS (:uV IgG and total antibodies. These 3 Methods used for detection were all not influenced by rheumatoid factor(RF).Conclusion All of the three Methods were specific and sensitive for the detection of specific antibodies to SARS-CoV , and useful for epidemiological research and clinical diagnosis, but not for early diagnosis of SARS.

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