文章摘要
贾崇奇,宁艳,刘同涛,刘兆兰.内皮型一氧化氮合成酶基因G894T变异与早发冠心病的关系[J].中华流行病学杂志,2005,26(1):51-53
内皮型一氧化氮合成酶基因G894T变异与早发冠心病的关系
Association between G894T mutation in endothelial nitric oxide synthase gene and premature coronary heart disease
收稿日期:2004-04-26  出版日期:2014-09-15
DOI:
中文关键词: 冠心病;一氧化氮合成酶,内皮型;基因
英文关键词: Cor onary heart disease;Nitr ic ox ide sy nthase, endo thelial;Gene
基金项目:
作者单位
贾崇奇 山东大学公共卫生学院流行病与卫生统计学研究所, 济南 250012 
宁艳 山东大学公共卫生学院流行病与卫生统计学研究所, 济南 250012 
刘同涛 山东大学齐鲁医院心内科, 济南 250012 
刘兆兰 山东大学公共卫生学院流行病与卫生统计学研究所, 济南 250012 
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中文摘要:
      目的探讨内皮型一氧化氮合成酶基因第7外显子G894T变异(Glu298Asp)与早发冠心病之间的关系。方法以医院为基础的病例对照研究,选择新诊断的冠心病患者为研究对象。男性55岁以前及女性65岁以前患冠心病为早发冠心病。以132例早发冠心病患者为病例组,172例迟发冠心病患者为对照组。运用PCR限制性片段长度多态性检测G894T变异。结果G894T变异基因型频率早发冠心病组(TT、GT、GG频率分别为6.06%、20.45%、73.48%)显著高于迟发冠心病组(TT、GT、GG频率分别为1.74%、11.63%、86.63%)(P=0.01)。T等位基因频率早发冠心病组(16.29%)也显著高于迟发冠心病组(7.56%)(P=0.001),OR=2.38,95%CI:1.38~4.16。在α=0.05显著性水平上,用多元逐步非条件logistic回归调整性别、吸烟、饮酒、超重后,G894T变异对早发冠心病仍具有显著影响(P=0.01),OR=2.25,95%CI:1.19~4.26。结论eNOS基因G894T变异可能是早发冠心病发病的重要危险因素之一。
英文摘要:
      Objective ?? To assess the association betw een G894T ( Glu298Asp) mutation in ex on 7 of t he endo thelial nitr ic ox ide sy nthase gene and premature coronar y heart disease ( P??CHD) . Methods Hospital??based case??control study w as conducted. New ly??diagnosed CHD patients were recruited as study subjects. 132 CHD patients diagnosed at/ befor e age 55 for males and 65 for females w er e assigned to P??CHD case g roup with other 172 CHD patients as the control gr oup. Polymer ase chain reaction with Ban ?? r estriction enzyme digestion w as performed to detect the G894T mutation. Results ?? G894T mutant g enotypes in P??CHD group ( TT, GT and GG fr equencies were 6. 06%, 20. 45% and 73. 48%, r espectiv ely) were significant higher than those in control group ( TT, GT and GG frequencies were 1. 74%, 11. 63% and 86. 63%, respectively ) ( P= 0. 01) . Mutant T allele frequency in P??CHD g roup w as also sig nificantly hig her than that in control gr oup ( 16. 29% v ersus 7. 56%, P = 0. 001, OR = 2. 38, 95% CI : 1. 38??4. 16) . Stepwise multiple logistic regression analysis at 0. 05 significant level w ith sex , smoking, alcohol dr inking, and overw eig ht covariates indicated that G894T mutation also hav ing significant effect on P??CHD ( P= 0. 01, OR = 2. 25, 95% CI : 1. 19?? 4. 26) . Conclusion ?? This study sug gested that G894T mutation in endothelial nitr ic ox ide sy nthase gene might serve as a major risk factor to the pathogenesis of P??CHD in this study population.
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