| 顾玲,汪华,朱凤才,史智扬,曾小燕,谭兆营,肖玉春,邱海燕,王鑫,金东,崔志刚,王冰,阚飙,王新生,景怀琦,徐建国.江苏省南通地区小肠结肠炎耶尔森菌分布特征初步研究[J].中华流行病学杂志,2005,26(10):786-789 |
| 江苏省南通地区小肠结肠炎耶尔森菌分布特征初步研究 |
| Study on the distribution of Yersinia enterocolitica in Nantong,Jiangsu province |
| 收稿日期:2005-01-06 出版日期:2014-09-15 |
| DOI： |
| 中文关键词: 小肠结肠炎耶尔森菌 血清型 毒力基因 |
| 英文关键词: Yersinia enterocolitica serotype Virulence gene |
| 基金项目:国家“十五”科技攻关计划资助项目(2003BA712A05-04)；江苏省“135”医学重点工程资助项目 |
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| 中文摘要: |
| 目的 了解江苏省南通地区小肠结肠炎耶尔森菌的分布特点和毒力特征。方法 用常规方法从家畜家禽粪便标本中分离小肠结肠炎耶尔森菌，PCR法检测其五种毒力相关基因，同时对国内分离到的O：8血清型菌株进行脉冲场凝胶电泳分析。结果 从该地区293份家畜家禽粪便标本中分离到91株小肠结肠炎耶尔森菌，分离率为31．06％。未分离到国内主要流行血清型O：3和O：9，其中有39．57％菌株毒力基因分布特征为：ail-、ystA-、ystB-、yadA-、virF-，有60．43％菌株为ail-、ystA-、ystB+、yadA-、virF-，两株国外引进的强毒菌株具有相同的脉冲图形，与国内分离到的O：8血清型菌株存在显著的差异。结论 致病性小肠结肠炎耶尔森菌的分布具有局限性，江苏省南通地区小肠结肠炎耶尔森菌的分布可能以非致病菌为主。 |
| 英文摘要: |
| Objective To study the distribution of Yersinia enterocolitica and its virulence factors in Nantong,Jiangsu. Methods Yersinia strains were isolated from livestock and poultry. Conventional PCR was used to detect the virulence factors of all strains and strains and strain 0:8 was analyzed by pulsed-field gel electrophoresis(PFGE). Results The combined isolatoin rate of Yersinia enterocolitica from livestock and poultry was 31.06% and the gene distribution characters ere:39.57% of them were ail-、ystA-、ystB-、yadA-、virF-; 60.43% were ail-、ystA-、ystB+、yadA-、virF- respectively. The two reference strains from America and Denmark showed similar electrophoresis patterns but were significantly different with 0:8 strains isolated from China while the serotypes of Yersinia enterocolitica 0:3 and 0:9 which were the main epidemic strains in China, were not found in this area. Conclusion The pathogenic Yersinia enterocolitis 0:3 and 0:9 were not found in Nantong,Jiangsu province. |
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