| 兰全学,扈庆华,石晓路,王冰,林一曼,张顺祥,程锦泉,阚飙,徐建国.深圳市1993-2002年霍乱弧菌的脉冲场凝胶电泳分析[J].中华流行病学杂志,2007,28(5):491-494 |
| 深圳市1993-2002年霍乱弧菌的脉冲场凝胶电泳分析 |
| Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993 to 2002 |
| 收稿日期:2006-08-31 出版日期:2014-09-18 |
| DOI： |
| 中文关键词: 霍乱弧菌 脉冲场凝胶电泳 分子分型 |
| 英文关键词: Vibrio cholerae Pulsed-field gel electrophoresis Molecular subtyping |
| 基金项目:国家自然科学基金资助项目(30300281),国家科技攻关计划(2003BA712A05-04),广东省卫生厅资助项目(A2003709) |
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| 中文摘要: |
| 目的 分析深圳市1993-2002年霍乱弧菌菌株的相关性。方法 60株霍乱弧菌基因组DNA经Not Ⅰ酶切，通过脉冲场凝胶电泳获得电泳图谱，利用BioNumerics软件对图谱进行聚类分析。结果 60株霍乱弧菌被分为39种脉冲场凝胶电泳图谱，聚类分析将大部分菌株分为A、B两群。结论 深圳地区霍乱弧菌存在紧密相关的流行克隆群。霍乱弧菌的脉冲场凝胶电泳分析，有助于霍乱的主动监测和传染来源追踪。 |
| 英文摘要: |
| Objective To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002. Methods. Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not 1 and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis(PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software. Results 39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B. Conclusion The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae. |
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