文章摘要
柴利泉,李丽,白大鹏,张立,邵世峰,吴琦,陆伟,孙照刚,李传友,李卫民,戴宗甲.天津地区临床分离结核分枝杆菌分型的初步研究[J].中华流行病学杂志,2007,28(8):785-788
天津地区临床分离结核分枝杆菌分型的初步研究
Study on the genotype of Mycobacterium tuberculosis isolates from hospitals in Tianjin
投稿时间:2007-01-25  修订日期:2007-08-10
DOI:
中文关键词: 结核分枝杆菌;北京基因型;北京分化支;W菌/典型北京家族菌株;非典型北京菌株
英文关键词: Mycobacterium tuberculosis;Beijing genotype;Beijing lineage;W strain/typical Beijing family strains;Atypical Beijing strains
基金项目:北京市自然科学基金资助项目(5062016);北京市优秀人才培养专项经费资助项目
作者单位E-mail
柴利泉 天津市海河医院结核病科, 300350  
李丽 天津市海河医院结核病科, 300350  
白大鹏 天津市海河医院结核病科, 300350  
张立 天津市海河医院结核病科, 300350  
邵世峰 天津市海河医院结核病科, 300350  
吴琦 天津市海河医院结核病科, 300350  
陆伟 天津市海河医院结核病科, 300350  
孙照刚 北京市结核病胸部肿瘤研究所  
李传友 北京市结核病胸部肿瘤研究所 lichuanyou6688@hotmail.com 
李卫民 北京市结核病胸部肿瘤研究所  
戴宗甲 北京市结核病胸部肿瘤研究所  
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中文摘要:
      目的 探讨天津地区结核分枝杆菌临床分离株分子流行病学特征.方法 连续收集天津市海河医院2005年8月16日-11月25日就诊患者痰培养阳性的结核分枝杆菌100株,采用间隔区寡核苷酸分型(spoligotyping)和多位点可变串联重复序列(VNTR)两种方法进行基因分型,并运用软件对二者的结果进行分析.依据北京分化支的定义,运用多重和实时定量PCR方法将其区分为W菌/典型北京家族菌株和非典型北京菌株,χ2检验分析两种亚群与患者年龄和耐药性之间的联系.结果排除污染菌株,共对96株结核分枝杆菌临床分离株进行两种方法的基因分型,spoligotyping结果 91.7%为北京基因型(含3株类北京基因型)结核分枝杆菌(88/96).VNTR分型可将北京基因型分为60种基因型.在北京分化支结核分枝杆菌中,W菌/典型北京家族菌株占93.2%(82/88).两种北京分化支亚群与患者年龄及耐药性之间差异无统计学意义(P>0.05).结论 天津地区结核病患者临床分离的结核分枝杆菌中,北京基因型呈现较为明显的优势.VNTR的分辨率明显高于spoligotyping.北京分化支的两种亚群在天津地区临床结核病患者中均有流行,但以W菌/典型北京家族菌株为主.
英文摘要:
      Objective To explore the characteristics on molecular epidemiology of Mycobacterium tuberculosis isolates from hospitals in Tianjin area.Methods One hundred M.tuberculosis isolated strains were collected in succession from August 16~(th)-December 25~(th),2005 in Tianjin Haihe Hospital and genotyped by spoligotyping and multiple locus variable number-tandem repeat(VNTR).Data was analyzed by cluster software.Based on the concept of Beijing lineage,it was determinate two sub-groups:atypical Beijing strains and W strain/typical family strains by multiplex and real-time PCR.The associations of sub- groups with drug resistance and age were assessed by the x~2 test.Results 96 M.tuberculosis strains were genotyped in which 91.7 %(88/96)strains belonged to Beijing genotype(including 3 Beijing-like strains) by spoligtyping.VNTR typing could differentiate 60 genotypes among the 88 Beijing genotype strains. 93.2% of the Beijing lineage M.tuberculosis strains of this study belonged to W strain/typical Beijing family strains(82/88).No statistically significant differences were observed in the proportions of the two sub-groupa in patients of different age,or drug resistance(P0.05).Conclusion The M.tuberculosis Beijing genotype strains were dominated on tuberculosis hospital patients of Tianjin ares.The discriminatory power of VNTR typing was higher than that of spoligtyping.The two sub-groups of Beijing lineage had been prevalent in Tianjin,however W strain/typical Beijing family strains were of preponderance.
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