文章摘要
冯燕,卢亦愚,严菊英,姜晓慧,史雯,徐昌平,李榛.不同人群血清对麻疹疫苗株与流行株的中和能力比较[J].中华流行病学杂志,2007,28(11):1123-1126
不同人群血清对麻疹疫苗株与流行株的中和能力比较
Study on the neutralization capacity of different types of human measles virus vaccine and the epidemic strains
投稿时间:2007-06-21  
DOI:
中文关键词: 麻疹病毒;中和抗体;抗原变异;几何平均滴度
英文关键词: Measles virus;Neutralization antibody;Antigenic difference;Geometric mean titer
基金项目:浙江省自然科学基金资助项目(Y204458)
作者单位
冯燕 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
卢亦愚 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
严菊英 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
姜晓慧 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
史雯 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
徐昌平 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
李榛 浙江省疾病预防控制中心病毒研究所, 杭州, 310009 
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中文摘要:
      目的探讨不同人群血清对麻疹流行株与疫苗株中和能力的差异。方法采集麻疹患者急性期与恢复期血清、麻疹疫苗免疫前后儿童血清以及流动人口血清,分别对疫苗株沪191与2005年麻疹流行株进行中和抗体(NT)滴度测定。同时,利用疫苗株、浙江省当地流行株制备动物免疫血清,与相应毒株进行交叉中和试验,测定各毒株之间抗原比。结果疫苗免疫后儿童血清对疫苗株的中和抗体几何平均滴度(GMT)为50.82,高于流行株MVi/zJ/05/7(GMT值为27.35)1.86倍;患者恢复期血清对流行株的中和能力(GMT值为386.95)显著高于疫苗株(GMT值为151.83);流动人口血清对流行株中和抗体GMT值均小于疫苗株,差异为2.22~4.17倍;Mvi/zJ/99/1、Mvi/ZJ/04/1和Mvi/ZJ/05/7与疫苗株间抗原比分别为4.28、5.24和5.66。此外,部分患者急性期血清对疫苗株存在低滴度的中和抗体,GMT值1:4左右。结论不同人群血清对麻疹疫苗株与流行株的中和能力的差异有统计学意义,低滴度的疫苗抗体不能有效保护个体免受麻疹流行毒株的侵袭,应加强麻疹病毒变异与疫苗效果的相关研究。
英文摘要:
      objective To explore the neutralization capacities of di“erent typeS of human serum tomeaSles virus epidemic strains and vaccine strain.methods Neutralization antibody(NT)to Shanghai 191and measles virus iSolates in 2005 were teSted using acute and∞nvalescent serum samples from diagnosedmeaSles patients,children serum samples collected before and after vaccination and serum samples of migrantreSidents,from 3 different regions. Additionally,animal immune serum referring to vaccine strain and 3epidemic strains were prepared and used to undergo crossing neutralization teSt with corresponding strainsmentioned-above.Antigenic ratios were calculated.Reults GMT value of NT of after-immune serum tovaccine strains was 50.82,1.86 times higer than that to Mvi/zJ/05/7(GMT was 27.35),whereas GMTvalue of convalescent serum to MVi/zJ/05/7(GMT was 386.95)was obviously higher than that to vaccinestrain(GMT was 1:151.83),and GMT value of migrant residents’serum in 3 regions to MVi/ZJ/05/7were 2.22-4.17 times 10wer than that to vaccine strain.Meanwhile,the antigenic ratios between MVi/zJ/99/1,Mvi/zJ/04/1,Mvi/ZJ/05/7 and vaccine strain were fOund to be 4.28,5.24 and 5.66 respectively.Additionally,low NT titerS to vaccine strain were found in patients’ acute sera and GMT value was over1:4.ConcIusion There were obvious differences on neutralization antibody of different types of serum tomeasles vaccine strain and epidemic strains which indicating the antigenic diversity of epidemic strains hadinfluencdd the protective effectiveness of vaccine antibody to epidemic strains. It was of significance to carryon research prOjects on the antigenic diversity and effectiveness of measles vaccine.
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