文章摘要
王丽丽,夏胜利,胡万富,顾玲,杨晋川,陈倩,崔志刚,许彦梅,王鑫,叶长 芸,景怀琦,徐建国.中国部分地区大肠埃希菌O157的分子分型及变异研究[J].中华流行病学杂志,2008,29(1):55-58
中国部分地区大肠埃希菌O157的分子分型及变异研究
Molecular epidemiology of enterohaemorrhagic Escherichia coli O157 in some areas in China
收稿日期:2007-07-05  出版日期:2014-09-18
DOI:
中文关键词: 大肠埃希菌O157;脉冲场凝胶电泳;聚合酶链反应
英文关键词: Human rhinovirus;Acute respiratory infections;Infants and young children
基金项目:北京市自然科学基金基础性研究实验室项目(JS96004);北京市优秀人才培养专项经费(20042D0300935);北京市科技新星计划项目资助(2006A63)
作者单位E-mail
王丽丽 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206 yqianbjc@263.net 
夏胜利 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
胡万富 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
顾玲 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
杨晋川 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
陈倩 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
崔志刚 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206  
许彦梅 河南省疾病预防控制中心  
王鑫 安徽省疾病预防控制中心  
叶长 芸 江苏省疾病预防控制中心  
景怀琦 江苏省徐州市疾病预防控制中心  
徐建国 北京市疾病预防控制中心  
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中文摘要:
      目的 了解出血性大肠埃希菌(EHEC)O157的分子流行特征和遗传变异关系并完善EHEC O157:H7感染性腹泻监测制度.方法 采用聚合酶链反应分析毒力基因的分布情况|用脉冲场凝胶电泳技术对1988-2005年部分地区分离到的249株EHEC O157:H7(其中产志贺毒素的245株,不产志贺毒素的4株)和51株O157非H7进行分子流行病学分析.结果 stx2基因在我国的EHEC O157:H7菌株中有着很高的分布率,部分菌株携带stx2基因变种.300株O157共分为161种带型,其中51株O157非H7菌株共有42种带型,4株O157:H7非产毒株分别为4种带型,245株EHEC O157:H7共有115种带型.结论 EHEC O157间的基因变异较大|产stx2原毒素的菌株和stx2毒素发生变异的菌株带型相差较大.部分菌株之间存在着一定的交叉,说明虽然这两大类菌株有其特有的流行克隆系,但是它们的克隆系之间亲缘关系较近。
英文摘要:
      Objective To understand the relationship between human rhinovirus(HRV) and acute respiratory infections in infants and young children in Beijing.Methods Throat swab/nasopharyngeal aspirates were collected from 3292 infants and young children with acute respiratory tract infections in Beijing from November 2002 to November 2006.Primers derived from the highly conserved 5'-noncoding region of human rhinovirus were used to detect HRV from clinical specimens by nested RT-PCR for which the sensitivity and specificity had been determined previously.Results Out of these 3292 specimens,507 were (15.4 %,507/3292) HRV positive with RT-PCR method.HRV were detected from 220 out of 1315 outpatients and 287 out of 1977 inpatients with positive rates as 16.7% and 14.5% respectively.HRV was detected from 50.0% (8/16) of the patients with pharyngitis.Among 280 specimens collected from patients with acute bronchitis,43 (15.4%) were HRV positive,including 14 from 80 patients with wheezy bronchitis (17.5%).High positive rates were also found in apeeimens from patients with pneumonia (12.6%,150/1189),bronchiolitis (16.0%,42/262) and asthma (12.8%,10/78).In 53 patients with initial diagnosis as hematic disease or other complicate respiratory infections,14 were HRV (26.4%,14/53) positive.As for the seasonal distribution,HRV were detected in most of the months during thie period of research.The highest positive rate of HRV in each year fell in September (32.6%),February (24.2%) of 2004,February of 2005 (35.3%) and March (31.3%) from 2003 to 2006,respectively.Among these HRV positive patients,44.8% were under 1 year of age (227/507),15.4%(78/507) were 1 to 2 years old and 12.4%(63/507) were 2 to 3 years old.Conclusion HRV was associated with acute upper respiratory infections and lower respiratory infections including bronchitis,pneumonia and bronchiolitis in pediatric patients.Patients with lower immunity such as those with hematic diseases,were more susceptible to be infected by HRV.HRV could be detected in all age groups in this study,but the positive rates were decreasing with the increase of patients' age.Infants under 1 year of age seemed to be more likely to get HRV infection.
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