文章摘要
姚亚萍,辛若雷,何翔,孟哲峰,潘晓红,郭志宏,杨介者,徐云,邢辉.浙江省HIV-1 CRF01_AE流行株的分子特征分析[J].中华流行病学杂志,2008,29(2):161-165
浙江省HIV-1 CRF01_AE流行株的分子特征分析
Molecular characteristics of HIV-1 CRF01_AE strains in Zhejiang province
投稿时间:2007-08-30  修订日期:2012-06-01
DOI:
中文关键词: 人类免疫缺陷病毒;分子特征;基因
英文关键词: Human immunodeficiency virus-1;Molecular characteristics;Genes
基金项目:湖南省农村公共卫生综合试点基金资助项目
作者单位
姚亚萍 浙江省疾病预防控制中心艾滋病性病防治所, 杭州 310009 
辛若雷 中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室 
何翔 中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室 
孟哲峰 中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室 
潘晓红 浙江省疾病预防控制中心艾滋病性病防治所, 杭州 310009 
郭志宏 浙江省疾病预防控制中心艾滋病性病防治所, 杭州 310009 
杨介者 浙江省疾病预防控制中心艾滋病性病防治所, 杭州 310009 
徐云 浙江省疾病预防控制中心艾滋病性病防治所, 杭州 310009 
邢辉 中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室 
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中文摘要:
      目的 分析艾滋病病毒1型(HIV-1)CRF01_AE重组毒株在浙江省的分子流行特征.方法 提取234例HIV-1感染者基因组DNA用巢式聚合酶链反应(nPCR)方法扩增gag部分基因片段,测定序列以后分析核苷酸和氨基酸序列.结果 在扩增阳性并成功测序的192个序列中HIV-1CRF01 AE有81个,占42%,是浙江省HIV主要的流行毒株之一.系统进化树中存在3个bootstrap值>60的分支簇,其中MIX组包括47个序列,内部基因离散率为2.4%±0.7%,与参考株CM240的基因离散率为3.4%±0.5%;SEX组包括7个样本,组内基因离散率为3.0%±1.2%,与参考株的基因离散率为3.9%±1.0%;MSM组包括12个样本,组内基因离散率为2.8%±1.1%,与参考株的基因离散率为4.9%±0.6%,三者差别有统计学意义.特征性氨基酸分析表明MIX组、SEX组、MSN组共享序列与国际参考株共享序列之间分别存在2个、4个和8个位点的差异.结论 浙江省内HIV-1 CRF01 AE流行株中主要存在三个进化簇,其中两个簇与广西来源株密切相关.CRF01_AE毒株在浙江省内主要以异性性途径和注射吸毒形式传播.
英文摘要:
      objective Using molecular epidemiology method to characterize human immunodeficiency virus type 1 (HIV-1)subtype CRFOl_AE strains being prevailed in Zhejiang province.Methods Gag fragments of the HIV-1 strains were amplified by nested-polymerase chain reaction (nPCR) from the DNA extracted from whole blood of HIV-1 infected individuals in Zhejiang province.PCR products were sequenced and analyzed by phylogenetic method.Results 81 HIV-1 subtype CRFO1_AE sequences were identified from the 192 samples that sequenced successfully.As one of the dominant subtypes in Zhejiang, CRFOl_AE was transmitted mainly by heterosexual or homosexual contact in local residents.In migrants living in Zhejiang, CRF01_AE were transmitted mainly by heterosexual contact or injecting drug use.There were three main clusters in the phylogenetic tree which bootstrap value was larger than 60.We named the clusters with group MIX (47 sequences),group SEX (7 sequences) and group MSM (12 sequences) based on the transmission.Pairwise DNA distances in the gag region within the three groups and between CM240 were different (P=0.000).Data through the analyses of deduced amino acid sequences from the three groups showed that several signature amino acid sites were distinct from the same positions of the subtype reference strains.Conclusion The CRFO1_AE strain prevailing in Zhejiang province was from several sources, transmitted by more than three different transmission routes, and becoming the main subtypes circulating in homosexual population in this study.More attention needs to be paid to the epidemic characteristic of CRFO1_AE.
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