| 谢翔,马依彤,付真彦,杨毅宁,王迎洪,陈邦党,刘芬.环氧化酶-2基因-765G＞C和前列环素合酶基因C1117A多态性与新疆维吾尔族人群心肌梗死的相关性研究[J].中华流行病学杂志,2008,29(6):598-603 |
| 环氧化酶-2基因-765G＞C和前列环素合酶基因C1117A多态性与新疆维吾尔族人群心肌梗死的相关性研究 |
| Study on the association of cyclooxygenase-2-765G＞C and prostacyclin synthase C1117A polymorphisms and the risk of myocardial infarction in Uigur population of Xinjiang,China |
| 收稿日期:2008-02-16 出版日期:2014-09-18 |
| DOI：10.3321/j.issn:0254-6450.2008.06.020 |
| 中文关键词: 心肌梗死|基因多态性|维吾尔族 |
| 英文关键词: Myocardial infarction|Gene polymorphism|Uigur |
| 基金项目:新疆维吾尔自治区高校科研计划创新群体基金资助项目(XJEDU 2005 G03) |
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| 目的 探讨环氧化酶-2(COX-2)基因-765G＞C和前列环素合酶(PGIS)基因C1117A多态性与新疆维吾尔族人群心肌梗死的相关性．方法 采用聚合酶链反应-限制性片段长度多态性方法，对新疆维吾尔族178例心肌梗死患者和175名健康体检者COX-2基因-765G＞C和PGIS基因C1117A多态性进行检测，同时进行血清6-酮-前列环素F1α水平及其他生化指标测定．结果 (1)PGIS基因C1117A不同基因型在心肌梗死组和健康对照组中频率分别为:CC型75.84%和64.57%，CA型17.42%和28.29%，AA型6.74%和9.14%，两组基因型和等位基因频率差异具有统计学意义(P＜0.05);(2)COX-2基因-765GG基因型在心肌梗死组为78.65%，明显高于对照组(55.43%)，差异具有统计学意义(P＜0.01)，但-765GC和-765CC基因型在心肌梗死组分布频率(19.66%和1.69%)明显低于健康对照组(34.86%和9.71%)，差异具有统计学意义(P＜0.05或P＜0.01)，且等位基因频率差异亦有统计学意义(P＜0.01);(3)联合基因分析显示，心肌梗死组PGIS基因1117CC基因型+COX-2基因-765GG基因型频率显著高于对照组(P＜0.01)，具有该联合基因型者发生心肌梗死的风险(OR=3.87)明显高于单独具有PGIS基因1117CC基因型(OR=1.72)或COX-2基因-765GG基因型(OR=2.94)者;(4)心肌梗死组6-酮-前列环素F1α水平较对照组降低，差异具有统计学意义(P＜0.05);在PGIS基因C1117A不同基因型之间及COX-2基因-765G＞C不同基因型之间，6-酮-前列环素F1α水平的差异无统计学意义(P＞0.05);但具有COX-2基因-765GG+PGIS基因1117CC联合基因型者6-酮-前列环素F1α水平明显降低于其他基因型，差异具有统计学意义(P＜0.05)．结论 PGIS基因CC基因型和C等位基因及COX-2基因-765GG基因型和G等位基因与新疆维吾尔族人群心肌梗死的发生具有相关性;携带COX-2基因-765GG+PGIS基因CC联合基因型者发生心肌梗死的风险显著增加，可能和该联合基因型导致的血清前列环素水平降低有关;COX-2基因-765CC基因型和C等位基因可能是新疆维吾尔族人群心肌梗死发生的保护因子． |
| 英文摘要: |
| Objective The purpose of this study was to investigate the association of genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase with myocardial infarction (MI)in Uigur population in Xinjiang. Methods 178 patients with MI and 175 healthy control subjects were detected on the genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase by polymerase chain reaction-based restriction fragment length polymorphism. Other serum 6-keto-PGF1α concentration and biochemical indicators were detected in all the subjects. Results (1)The genotype distributions of the control group and MI group were in the Hardy-Weinberg equilibrium. The frequencies of CC, CA and AA genotype of prostacyclin synthase were 75.84%, 17.42% and 6.74% in MI group while they were 64.57%, 28.29% and 9.14% in controls respectively. There was significant difference in frequencies of CC genotype and C allele as well as CA and AA genotypes between controls and MI cases. (2)The frequencies of -765GG,-765GC and -765CC genotype of cyclooxygenase-2 were 78.65%, 19.66% and 1.69% in MI group while they were 55.43%, 34.86% and 9.71% in controls respectively. There was significant difference in frequencies of three genotypes and alleles between the two groups (P＜0.05 or P＜0.01 ). (3) In combined genotype analysis, the genotype of PGIS CC + COX-2 -765GG was significantly higher in patients with MI than in control subjects (P＜0.05). The odds ratio estimated through combined analysis of the PGIS CC and COX-2 -765GG genotypes(OR=3.87) markedly increased when compared with that estimated separately from the PGIS CC ( OR=1.72 ) or COX-2 -765GG ( OR= 2.94 ) genotype. (4)There was a significant difference in serum 6-keto-PGF1α level between MI group and control group (P＜0.05 ), but there were no differences found in every genotype of PGIS and COX-2 gene (P＞0.05 ). In the cases with both COX-2 -765GG and PGIS CC genotypes, the serum 6-keto-PGF1α levels was lower than that of others (P＜0.05). Conclusion The CC genotype and C allele of prostacyclin synthase, -765GG genotype and G allele of COX-2 might serve as risk factors of MI of Uigur population in Xinjiang.Populations with both COX-2 -765GG and PGIS CC genotypes were more at risk with MI than others which might be resulted from the decreased serum 6-keto-PGF1α concentration. The -765CC genotype and C allele of COX-2 gene might have protective functions on MI among Uigur population in Xinjiang. |
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