| 邓小玲,李柏生,谭海玲,孙立梅,柯碧霞,柯昌文,王多春,阚飙,钟豪杰.广东省2007年霍乱监测的病原特征分析[J].中华流行病学杂志,2008,29(7):696-699 |
| 广东省2007年霍乱监测的病原特征分析 |
| The etiologic characteristics of Vibrio cholerae inguangdong province in 2007 |
| 收稿日期:2008-03-06 出版日期:2014-09-15 |
| DOI： |
| 中文关键词: 霍乱弧菌 脉冲场凝胶电泳 优势菌型 |
| 英文关键词: Vibrio cholerae Pulsed fieldgel electrophoresis Predominated biotype |
| 基金项目:国家“ 863 ”髙技术研究发展计划资助項目 (2006AA02Z425);广东省医学科研指令性课题资助项目 (C2007002) |
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| 中文摘要: |
| 目的 分析2007年广东省霍乱弧菌分离株的病原学特征,比较不同地区流行优势菌型 之间以及霍乱疫情分离株与常规监测分离株之间的克隆相关性。方法 对疫情与监测菌株进行常规生物分型,利用脉冲场凝胶电泳技术,分别对不同地区优势菌型稻叶型Id之间、霍乱疫情与常规监测中分离的相同型别菌株之间进行分子指纹图谱的相似性分析,探讨菌株间的相关性。结果 2007年从广东省霍乱疫情中获得31株菌株,共3种血清型,优势菌型为01群稻叶型ld,不同地区病例的稻 叶型Id菌株分子分型相似度在94,5%?100%之间;常规监测分离株〗6株,菌型分布散在,与疫情菌株的菌型分布一致性差,相同生物型的霍乱疫情与监测菌株同源性不高。结论 广东省2007年霍乱 优势菌型稻叶型Id菌株为多克隆并存,显示为流行间歇期的特征。需利用分子分型技术开展分离株 的分析,加强对流行的预警监测 |
| 英文摘要: |
| Objective To analyze the etiologic characteristics of Vibrio cholerae inguangdong province in 2007.genetic relationship was observed including among predominated biotype isolates from different areas within the province and among same biotypes isolates from cholera cases and regular surveillance. Methods Isolates from cholera cases and through environmental surveillance were typed by sero- and phage- typings. Similarity of molecular fingerprinting was analyzed through comparing the pulsed fieldgel electrophoresis (PFGE) pattern of predominated biotype isolates, and those of the same biotype isolates from cholera cases and environment surveillance, respectively. In addition,genetic relationship was determined by clustering analysis, using bionumerics software. Results In total, 31 isolates from cholera cases were collected and subtyped for 3 serogroups. V. cholerae 01 El Tor Inaba phage ld was the predominant biotype which causing most of the cases inguangdong province in 2007. Data from cluster analysis showed that the similarity among Inaba phage ld strains from different areas were from 94.5%to 100%. However, 16 isolates were collected from environment surveillance programs and the predominated biotype could not be found. Additionally, the bio type distribution of cases isolates was not consistent with those isolates through surveillance. High phylogenetic diversity was observed for the same biotypes isolates from cases and surveillance samples. Conclusion Our data showed that V. cholerae 01 El Tor Inaba phage ld was the predominated biotype with multi-clone coexisting and circulating inguangdong province in 2007. It also appeared to be the characteristics of cholera in the non-epidemic period, suggesting that it was necessary to enhance the alert surveillance programs for cholera epidemic based on the molecular typing techniques. |
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