| 谢荣辉,付士红,程胤凯,徐芳,姚苹苹,翁景清,朱函坪,张意坚,朱智勇.浙江省蚊虫中乙型脑炎病毒的分离及鉴定[J].中华流行病学杂志,2008,29(7):712-715 |
| 浙江省蚊虫中乙型脑炎病毒的分离及鉴定 |
| Isolation and identification of Japanese encephalitis virus from mosquitoes in Zhejiang province |
| 收稿日期:2008-01-04 出版日期:2014-09-15 |
| DOI： |
| 中文关键词: 乙型脑炎病毒 病毒分离 序列分析 |
| 英文关键词: Japanese encephalitis virus Isolation of virus Sequence analysis |
| 基金项目:浙江省医药卫生科学研究基金资助项目(2008B39) |
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| 中文摘要: |
| 目的 对2007年浙江省采集的蚊虫标本进行病毒分离与鉴定。方法 2007年在浙江 省采集蚊虫标本,用金黄地鼠肾细胞(BHK-21)分离病毒,对新分离的病毒进行血清学和分子生物学 鉴定。结果 采集到4735只蚊虫,分离到2株致BHK-21细胞病变的病毒,经免疫荧光和RT-PCR试验 鉴定为乙型脑炎病毒(JEV),利用病毒PrM区段进行基因分型分析,新分离的2株病毒属于基因I型JEV。对这2株病毒的E基因区段进行分析,核苷酸和氨基酸的同源性分别为99.0%和98.8%。与疫苗株SA144-14-2相比,核苷酸同源性在87.7%,氨基酸同源性在96.4%以上,共有14个共同的氨基 酸位点差异。结论 在浙江省采集的蚊虫标本中分离到2株病毒,经鉴定为基因I型JEV,是浙江省近年来首次分离。 |
| 英文摘要: |
| Objective To study the situation of arboviruses carried by mosquitoes in Zhejiang province. Methods Mosquitoes were collected from Zhejiang province in 2007. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK-21 cell line. The isolated strains were identified by serological (IFA) and molecular methods (RT-PCR). Results Two strains were isolated from mosquitoes causing cytopathogenic effect (CPE) in BHK-21 cells. Results from serological tests showed that both of the two strains were positive for the antibody to Japanese encephalitis virus (JEV). PrM and Egene were then cloned and sequenced. Results from the phylogenetic analysis showed that the isolates belonged togenotype I JEV while through sequence analysis it showed that the homology of nucleotide sequences and amino acid sequences between the two strains were 99.0%and 98.8%respectively. Compared with the JEV vaccine strain SA14-14-2 and two strains, the homology of nucleotide sequences was up to 87.7%and homology of amino acid sequences was up to 96.4%. When comparing with the vaccine strain SA14-14-2, there were 14 common amino acid variations in all the two strains. Conclusion Two strains of JEV were isolated from mosquitoes collected in Zhejiang province which was the first isolation ofgenotype I JEV in the province in recent years. |
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