文章摘要
胡忠玉,何鹏,方鑫,邱少辉,梁争论,李河民,庄辉.不同种类乙型肝炎重组疫苗诱导免疫应答特点比较[J].中华流行病学杂志,2008,29(8):810-814
不同种类乙型肝炎重组疫苗诱导免疫应答特点比较
Comparison of the kinesis of immune responses in mice vaccinated by different kinds of recombinant hepatitis B vaccines
投稿时间:2008-01-24  修订日期:2012-05-28
DOI:
中文关键词: 乙型肝炎;疫苗;细胞因子;酶联免疫斑点法;斑点形成细胞;细胞毒T淋巴细胞反应
英文关键词: Hepatitis B;Vaccine;Cytokine;Enzyme-linked immunospot;Spot-forming cell;Lymphocytotoxicity
基金项目:国家。十五”科技攻关课题资助项目(2004BA718802)
作者单位E-mail
胡忠玉 中国药品生物制品检定所,北京100050 lzlun@yahoo.com 
何鹏 中国药品生物制品检定所,北京100050  
方鑫 中国药品生物制品检定所,北京100050  
邱少辉 中国药品生物制品检定所,北京100050  
梁争论 中国药品生物制品检定所,北京100050  
李河民 中国药品生物制品检定所,北京100050  
庄辉 北京大学医学部,北京100050  
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中文摘要:
      目的<\b> 比较三种乙型肝炎重组疫苗(rHB)[乙型肝炎汉逊酵母重组疫苗(rHP)、酿酒酵母重组疫苗(rSC)和CHO莺组疫苗(rCHO)]诱导小鼠细胞和体液免疫应答特点。方法<\b> 给3组小鼠(BALB/e,H.26)分别皮下接种3种r船3嵋,于免疫后第4、7、10、14、25和35 d分离脾单个核细胞(MNC),应用酶联斑点法(ELISPOT)测定MNCs体外刺激后所产生的细胞因子IFN.7、IL-2斑点形成细胞数(SFC)。刺激物为多肽或Hmhg。同时测定免疫后不同时间的细胞毒T淋巴细胞反应(CTL)活性和小鼠血清抗一HBs水平。结果<\b>应用ELISPOT法,不同种类rHB免疫小鼠后诱导分泌IFN一7和IL一2峰值均为10—14 d;rHP诱导CTL活性(P=0.000<0.05)和分泌IFN一7水平(P结论<\b>3种疫苗免疫小鼠的应答特点不同。酵母重组疫苗早期诱导细胞免疫应答优于rCHO;rCHO的优势是诱导产生抗一HBs早,且滴度较高。
英文摘要:
      Objective To evaluate the kinesis of cellular and humoral immune responses to differentkinds of recombinant hepatitis B(rHB) vaccines in the immunized mice. Methods At serial time points,the levels of IFN-γ and IL-2 secreted by spleeny mononuclear cells(MNC) of the vaccinated mice were detected by enzyme-linked immunospot methods(ELISPOT) after stimulation in vitro with FIBsAg MHC class I peptide S28.39 or HBsAg. The lymphocytotoxieity of the immunized mice were also detected (CTL) by a specific lysis assay and the levels of anti-HBs were measured by the Abbott IMX kit. Results The peak values of IFN-γ and IL-2 in vaccinated mice were detected by ELISPOT, 10-14 days after immunization. The CTL and the level of IFN-γ induced by rHB vaccine derived from yeast cells(Hansenular polymorpha) (rHP vaccine) were significantly higher than the other two vaccines (P<0.05). The maximum lysis of CTL appeared in the vaccinated mice on day 10 after immunization, with the percentage of 39.8 %. The levels of IL-2 induced by rHP vaccine were significantly higher than the other two vaccines (P<0.05). However,the IL-2 levels in the rSC (saccharomyces cerevisiae) vaccine group were higher as compared with the rCHO vaccine group at day 7 and day 14(7dt=4.595,P=0.001<0.05;14dt=5.721,P=0.000<0.05) after immunization. The cellular immune response to the rHP vaccine was thestrongest while it was the lowest to the rCHO vaccine at day 7 after immunization. The sero-positive rates and the titers of anti-HBs in the vaccinated mice increased with time after vaccination. The titers of anti-HBs in the rCHO vaccine group at day 7 were similar to the rSC vaccine group,but significantly higher than that of the rHP vaccine group(P=0.044<0.05). The anti-HBs titers of the rCHO vaccine group at day 14 were significantly higher as compared to the rSC(P=0.012<0.05 ) and rHP(P=0.009<0.05) vaccine groups. Conclusion The immune responses induced by the three kinds of rHB vaccines were different in their patterns and levels. According to the intensity of early cellular immune response, the two yeast HB vaccines were superior to the rCHO vaccine, especially to the rHP vaccine. In contrast, the rCHO vaccine induced early seroconversion and high levels of anti-HBs.Objective To evaluate the kinesis of cellular and humoral immune responses to differentkinds of recombinant hepatitis B(rHB) vaccines in the immunized mice. Methods At serial time points,the levels of IFN-γ and IL-2 secreted by spleeny mononuclear cells(MNC) of the vaccinated mice were detected by enzyme-linked immunospot methods(ELISPOT) after stimulation in vitro with FIBsAg MHC class I peptide S28.39 or HBsAg. The lymphocytotoxieity of the immunized mice were also detected (CTL) by a specific lysis assay and the levels of anti-HBs were measured by the Abbott IMX kit. Results The peak values of IFN-γ and IL-2 in vaccinated mice were detected by ELISPOT, 10-14 days after immunization. The CTL and the level of IFN-γ induced by rHB vaccine derived from yeast cells(Hansenular polymorpha) (rHP vaccine) were significantly higher than the other two vaccines (P<0.05). The maximum lysis of CTL appeared in the vaccinated mice on day 10 after immunization, with the percentage of 39.8 %. The levels of IL-2 induced by rHP vaccine were significantly higher than the other two vaccines (P<0.05). However,the IL-2 levels in the rSC (saccharomyces cerevisiae) vaccine group were higher as compared with the rCHO vaccine group at day 7 and day 14(7dt=4.595,P=0.001<0.05;14dt=5.721,P=0.000<0.05) after immunization. The cellular immune response to the rHP vaccine was thestrongest while it was the lowest to the rCHO vaccine at day 7 after immunization. The sero-positive rates and the titers of anti-HBs in the vaccinated mice increased with time after vaccination. The titers of anti-HBs in the rCHO vaccine group at day 7 were similar to the rSC vaccine group,but significantly higher than that of the rHP vaccine group(P=0.044<0.05). The anti-HBs titers of the rCHO vaccine group at day 14 were significantly higher as compared to the rSC(P=0.012<0.05 ) and rHP(P=0.009<0.05) vaccine groups. Conclusion The immune responses induced by the three kinds of rHB vaccines were different in their patterns and levels. According to the intensity of early cellular immune response, the two yeast HB vaccines were superior to the rCHO vaccine, especially to the rHP vaccine. In contrast, the rCHO vaccine induced early seroconversion and high levels of anti-HBs.
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