| 朱丹,曾志磊,彭丹,陈晓,赵立波,张英英,徐明鸣,展群岭,余建平,谢鹏.新疆伊犁地区马群中博尔纳病病毒自然感染调查[J].中华流行病学杂志,2008,29(11):1106-1109 |
| 新疆伊犁地区马群中博尔纳病病毒自然感染调查 |
| Epidemiological investigation on natural infection to Borna disease virus (BDV) among horses in Yili,Xinjiang |
| 收稿日期:2008-06-26 出版日期:2014-09-11 |
| DOI： |
| 中文关键词: 博尔纳病病毒 巢式RT-PCR TaqMan探针 马 |
| 英文关键词: Borna disease virus Nested RT-PCR TaqMan probe Horse |
| 基金项目:国家"863"高技术研究发展计划资助项目(2006AA022196) |
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| 中文摘要: |
| 目的 了解新疆伊犁地区马群中博尔纳病病毒(BDV)的流行状况,分析该病毒的种系来源.方法 采用改进的巢式反转录PCR(nRT-PCR)方法对新疆伊犁地区120匹马的外周血单个核细胞(PBMC)及脑组织中的BDV p24基因片段进行检测,对阳性产物进行基因序列测定和同源性分析.结果 有3匹马外周血和腩组织中同时检测到BDV,阳性率为2.5%(3/120).扩增产物序列与其他国外马源BDV分离株同源性>93%,与标准株He/80同源性达到98%以上.结论 新疆伊犁地区马群中存在BDV的自然感染.该地区BDV流行株与标准株He/80存在高度的同源性. |
| 英文摘要: |
| Objective To investigate the epidemiological pattern of Borna disease virus (BDV)infection in horses and to analyze the phylogenetic tree of derived BDV in Yili, Xinjiang. Methods We established a modified nested RT-PCR (nRT-PCR) to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) and brain tissues of 120 horses in Yili, Xinjiang. Positive products were analyzed by sequencing and homology analysis. Results The positive rate of BDV infection was 2.5% in both PMBCs and brain tissues at the same time. The gene sequence revealed in positive PCR samples was more than 93 %,identical to that of BDV derived from horses in other countries. We also noticed a high degree of identity ( >98 % ) to standard strain He/80 in gene sequence of positive PCR samples. Conclusion Our study found the presence of BDV natural infection in horses in Yili. The endemic BDV had a high degree of identity to standard strain He/80. |
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