文章摘要
潘浩,朱叶飞,祁贤,张永杰,李亮,邓斐,吴斌,王慎骄,朱凤才,汪华.江苏省2008年某福利院手足口病暴发的流行病学和病原学特征研究[J].中华流行病学杂志,2009,30(4):339-343
江苏省2008年某福利院手足口病暴发的流行病学和病原学特征研究
Analysis on the epidemiological and genetic characteristics of enterovirus type 71 and Coxsackie A16 virus infection in Jiangsu,China,2008
收稿日期:2008-10-29  出版日期:2014-09-17
DOI:
中文关键词: 手足口病;肠道病毒71型;柯萨奇病毒A组16型;序列分析
英文关键词: Hand-foot-and-mouth disease;Enterovirus type 71 ;Coxsackie A16 virus;Sequence analysis
基金项目:
作者单位
潘浩 上海市疾病预防控制中心 200338 
朱叶飞 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
祁贤 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
张永杰 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
李亮 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
邓斐 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
吴斌 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
王慎骄 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
朱凤才 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
汪华 江苏省疾病预防控制中心急性传染病防制科 210009, 南京 
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中文摘要:
      了解2008年江苏省某福利院手足口病(HFMD)暴发疫情的流行病学和病原学特征。方法对该福利院首起暴发疫情开展流行病学调查,并进行3个月的跟踪随访。实验方法采用荧光定量RT-PCR法检测肠道病毒71型(EV71)和柯萨奇病毒A组16型(CA16)病毒,并用RD、Vero细胞分离病毒,间接免疫荧光法鉴定分离株,并对EV71分离株的VPI基因序列进行种系发生分析。结果该福利院首起暴发疫情发病数为21例,罹患率为20.0%;10份粪便标本分离出3株EV71病毒,其VPI基因序列分析表明为CA基因亚型,与安徽省阜阳地区08株的同源性在97.9%~100.0%之间。随访研究发现EV71病毒在疫情第1周即可通过粪便排毒,最长排毒期近10周。首起疫情发生2个月后,该福利院又暴发产生6例CA16病毒感染的病例,两起疫情均有3例发生潜伏期排毒,7名婴幼儿被发现机体同时存在两种病毒。结论该福利院先后两起疫情分别由EV71和CA16病毒引起。EV71分离株为CA基因亚型,同安徽省阜阳地区08株有较高的同源性。两种病毒在机体中存在潜伏期排毒、混合感染(或携带)和超长排毒期现象。
英文摘要:
      determine the epidemiological features of hand-foot-and-mouth disease (HFMD) outbreaks and the genetic characteristics of enterovirus type 71 (EV71) isolates from patients in Lianyungang,Jiangsu province in May,2008.Methods Epidemiological,microbiological,cellular and molecular methods were performed to investigate pathogens and to describe the homogeneity of isolated strains.Results 21 cases were reported in this HFMD outbreak with the attack rate as 20.0%.3 EV71 virus strains were isolated from 10 stool samples.The nucleotide and amino acid homogeneity of these 3 Jiangsu strain with Anhui Fuyang strains were 97.9%-100.0% and 99.7%-100.0%,respectively.These 4 Jiangsu strains were within genotype C sub-geno group C4 in phylogenetic tree.Data from the follow-up study showed that shedding of EV71 and Coxsackie A 16 virus (CA 16) in the latent period appeared in the outbreak of HFMD.Human beings could be infected by both EV71 virus and CA16 at the same time and could also carry the two viruses.We also discovered that EV71 virus could be expelled out of the human body through stool in the fast week and last for 10 weeks.Conclusion The recently identified EV71 isolates from this HFMD outbreak belonged to sub-geno group CA.Facts as:the release of viruses in the latent period,co-infection or coexisting of two viruses at the same time and super long period of expulsion of toxin exist in EV71 and CA16 did exist.
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