| 董海燕,吕冰,张媛媛,刘志广,赵秀芹,蒋毅,万康林.中国结核分枝杆菌间隔区寡核苷酸分型方法标准化操作程序的探讨[J].中华流行病学杂志,2009,30(4):384-387 |
| 中国结核分枝杆菌间隔区寡核苷酸分型方法标准化操作程序的探讨 |
| Introduction to the standard operation program of Spoligotyping on Mycobacterium tuberculosis in China |
| 收稿日期:2008-09-24 出版日期:2014-09-17 |
| DOI： |
| 中文关键词: 结核分枝杆菌 间隔区寡核苷酸分型 标准化操作程序 北京家族 |
| 英文关键词: Mycobacterium tuberculosis Spacer oligonucleotide typing Standard operation program Beijing family |
| 基金项目:国家自然科学基金(30771853) |
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| 中文摘要: |
| 目的探讨中国结核分枝杆菌间隔区寡核苷酸分型(Spoligotyping)的标准化方法，初步评价其应用价值。方法采用核酸提取、聚合酶链反应(PCR)、反向线性点杂交等技术，结合BioNumeries(Version5.0)软件，对224株结核分枝杆菌临床分离株进行分型研究。结果使用Spoligotyping标准化方法对224株中国结核分枝杆菌临床分离菌株进行基因分型，将其分为北京家族菌株和非北京家族菌株两大簇。其中北京家族菌株129株，为主要的流行菌株。结论初步确定中国Spoligotyping标准化技术方案。该方法快速、简便、重复性好，能同时对结核分枝杆菌进行检测和分型，有利于结核病传染源的追溯和流行趋势的研究，尤其是在鉴定北京家族菌株上具有独特作用。 |
| 英文摘要: |
| study and preliminarily evaluate the standard spacer oligonucleotide typing (Spoligotyping) method and the application on Mycobacterium tuberculosis (M.tuberculosis).Methods Spoligotyping on 224 M.tuberculosis strains was studied by the molecular biological techniques,including DNA isolation,PCR,reverse line blot hybridization,together with data analysis software BioNumerics (Version 5.0).Results Standardization on both Spoligotyping method and parameters in result analysis and the usage of the analysis software were studied.Through this method,224 M.tuberculosis clinical strains were classified into 2 clusters including 129 Beijing family strains and 95 non-Beijing family strains.The predominant strains belonged to Beijing family.Conclusion Standard Spoligotyping method was preliminary determined in China,showing that it was a simple,rapid,and robust method for simultaneous detection and typing of M.tubereulosis.This method can be used for tracing the source of infection and understanding the epidemic trend of M.tuberculosis.Spoligotyping can also be served as a method for simultaneous detection and typing ofM.tuberculosis,and to identify Beijing family strains. |
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