文章摘要
杨静静,张云,邓小昭,许可,王忠灿,王洁,冯乐,丁伟良.丙型肝炎病毒基因1b型F蛋白对人肝细胞癌细胞株凋亡的影响[J].中华流行病学杂志,2009,30(4):388-392
丙型肝炎病毒基因1b型F蛋白对人肝细胞癌细胞株凋亡的影响
Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinom cell apoptosis
收稿日期:2008-09-22  出版日期:2014-09-17
DOI:
中文关键词: 丙型肝炎病毒;F蛋白;凋亡;肿瘤坏死因子α
英文关键词: Hepatitis C virus;F protein;Apoptosis;Tumor necrosis factor α
基金项目:江苏省高校自然科学基金(03KJD330144)
作者单位
杨静静 南京医科大学公共卫生学院 210029 
张云 南京医科大学公共卫生学院 210029 
邓小昭 南京军区军事医学研究所 
许可 江苏省疾病预防控制中心 
王忠灿 南京军区军事医学研究所 
王洁 南京医科大学公共卫生学院 210029 
冯乐 江苏省宜兴市人民医院 
丁伟良 江苏省宜兴市人民医院 
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中文摘要:
      目的研究丙型肝炎病毒(HCV)基因1b型F蛋白对人肝细胞癌细胞株凋亡的影响。方法构建真核表达重组质粒pcDNA3.0-F-EGFP,并利用Lipofectamine2000转染人肝细胞癌细胞系,同时设pcDNA3.0-C-EGFP-阳性对照、pcDNA3.0-HcpG2为阴性对照及未转染为空白对照,以Act-D、TNFα诱导四组细胞系发生凋亡,AnnexinV-FITC/PI双染检测肿瘤细胞凋亡率,并使用细胞凋亡DNALadder检测,进一步验证HCV1b型F蛋白在肝细胞凋亡的生物学功能效应。结果pcDNA3.0-F-EGFP-细胞系在发生凋亡的时间上比peDNA3.0-C-EGFP-快,但相对空白质粒转染组及未转染细胞系有明显延迟,凋亡的发生率也明显低于阴性对照及空白对照细胞系(P<0.001)。结论外源性基因HCV1b型F的引入及表达,对细胞的凋亡有抑制作用。
英文摘要:
      investigate the effects of F protein of hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom cells.Methods cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP- strain was exposed to Act-D and tumor necrosis factor a (TNFα) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-,negative control pcDNA3.0-C-EGFP- and blank control.Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells.DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotie cells.Results pcDNA3.0-F-EGFP- cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0- strain and strain (P<0.001).Conclusion The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1b) could significantly inhibit the apoptosis of cells.
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