| 胡永峰,赵蓉,金奇.抗真菌药物靶点研究现状[J].中华流行病学杂志,2009,30(8):857-859 |
| 抗真菌药物靶点研究现状 |
| Current situation of the research for antifungal drug's targets |
| 收稿日期:2009-01-20 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 抗真菌药物 靶点 |
| 英文关键词: Antifungal drug Targets |
| 基金项目:国家自然科学基金(30671799,30872159);国家"863"计划课题(2006AA02Z 402):上海市重点学科建设项目(B118) |
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| 中文摘要: |
| 真菌可引起人类慢性传染病,发病率高,在世界各地广泛流行.由真菌感染引起的真菌病可分为浅部真菌病和深部真菌病(又名系统性真菌病).皮肤癣菌(例如红色毛癣菌)感染引起的是浅部真菌病,包括头癣、股癣、甲癣等. |
| 英文摘要: |
| Objective To optimize the serum bactericidal assay (SBA), detect and analyze the bactericidal antibody level against Neisseria meningitidis serogroup C strains after divalent polysaccharide (A plus C) vaccine immunization. Methods Two Neisseria meningitidis serogroup C strains, vaccine candidate strain (C11) and epidemic strain (053442), were selected as targets. The national Neisseria meningitidis standardized serum was used as reference serum. Pel-Freez infant rabbit complements was available. The optimized SBA method was used to detect bactericidal antibody against strain C11 and 053442 for 122 pairs of sera before and after immunization with a divalent polysaccharide (A and C) vaccine. Results The strain C11 and 053442 both could be used as targeted strain for SBA. The optimized concentration of targeted strain was achieved when a whole-cell suspension of 0.35 A at 600 nm was diluted 4×104 times. Before immunization, SBA geometric mean titers(GMT) of 122 sera against strain C11 and 053442 were 1: 1.75 and 1:2.63 respectively, and the protective rates were 9.8% and 17.2% respectively. After immunization, the GMTs and the protective rates of 122 sera both rose significantly (P<0.01), the GMTs against strain C11 and 053442 were 1:483.73 and 1:412.57 respectively. The protective rates against strain C11 and 053442 were 100% and 95.9% respectively. Conclusion Immunization with a divalent polysaccharide (A and C) vaccine could elevate remarkably the population SBA titer against Neisseria meningitidis serogroup C strains of different subtypes, but the surveillance of vaccine effect against different targeted strains remains necessary. |
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