文章摘要
李一冰,乌兰娜,刘志红,李鹃,王纯,周艳秋,吴瑞芳.自然人群子宫颈脱落细胞中人端粒酶RNA组份基因的表达及临床意义[J].中华流行病学杂志,2009,30(9):946-950
自然人群子宫颈脱落细胞中人端粒酶RNA组份基因的表达及临床意义
Amplification and clinical significance of hTERC gene in the eervicaI exfoliated cells fromnaturaI population in Shenzhen
投稿时间:2009-03-03  修订日期:2007-12-06
DOI:
中文关键词: 子宫颈癌;子宫颈上皮内瘤变;荧光原位杂交;人端粒酶RNA组份;液基细胞学
英文关键词: Cervical cancer;Cervical intraepithelial neoplasia;Fluorescence in situ hybridization;Human telomerase RNAcomponent;Liquid.based cytology
基金项目:军队"十一五"课题资助项目(06MA294)
作者单位E-mail
李一冰 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
乌兰娜 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
刘志红 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
李鹃 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
王纯 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
周艳秋 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036  
吴瑞芳 北京大学深圳医院妇产科子官颈癌早诊早治中心, 深圳 518036 wttrfl00@126.com 
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中文摘要:
      目的调查深圳市人群宫颈脱落细胞中人端粒酶RNA组份(hTERc)基因的表达,探讨其与人乳头瘤病毒(HPV)感染、宫颈脱落细胞学异常及宫颈上皮内瘤变(cIN)之间的关系。方法以深圳市某社区30~59岁智力正常的妇女388人为研究对象,同步进行宫颈脱落细胞的液基细胞学检查、HC.II法高危型HPV(HR.HPv)检测和荧光原位杂交(FISH)法hTERC基因检测。对于细胞学为ASCUS及以上病变,及/或HR-HPV阳性者,及/或hTERC有扩增者均行阴道镜下宫颈四象限多点活检并病理诊断。结果经病理确定CIN I、Ⅱ、Ⅲ及宫颈癌例数分别为55(14.18%)、4(1.03%)、9(2.32%)和1(0.26%),宫颈脱落细胞的hTERC扩增率为8.76%。hTERC扩增在有/无HPV感染及不同细胞、组织学类型者存在差别:(1)HPV阳性率为17.01%;hTERC扩增在HPV阳性与阴性组分别为19.70%与6.52%,两组比较差异有统计学意义(P<0.01)。(2)hTERC扩增在细胞学无宫颈上皮内瘤变(NILM)组为5.97%、ASCUS 18.75%、LSIL 10.00%、ASC-H66.67%、HSIL 100.00%;在HSIL及以上病变组扩增率明显升高(P结论HTERC的扩增与宫颈细胞学和组织学异常密切相关,其扩增率随宫颈病变程度的加重而增加,hTERC扩增与否有可能作为判断有无高度宫颈上皮内瘤变及估计预后的指标之一。
英文摘要:
      Objective To study the amplification of human telomerase RNA component(hTERC)gene in the cervical exfoliated cells from natural population in Shenzhen and to explore its relationship with human pappiloma-virus(HPV)infection.abnormal cervical cytology and cervical intraepithelial neoplasia(CIN).Methods Tllrce hundred and eighty.eight women.30-59 year-old and having normaI intelligence in a community of Shenzhen.were examined with liquid-based cytology.Human pappilorna-virus(HR.HPV)was tested by hybrid capture lI(HC.II)and hTERC gene detection by fluorescence in situ hybridization(FISH).Patients with ASCUS and above lesion,and/or positive HR-HPV results and/or amplification of hTERC gene were examined by colposcopy,multiple biopsies of cervical quadrant and pathology.Results The frequencies of CIN I,Ⅱ,Ⅲ,cervical cancer were 55(14.18%),4(1.03%).9(2.32%)and 1(0.26%)respectively,with the rate of hTERC gene amplification as 8.76%.There were significantly differences of hTERC amplification among the samples with different cytological and histologicallesions as well witll HPV infection(P<0.O 1).(1)The positive rate of HPV infection was l7.0 1%;the positive rates of hTERC gene amplification were 19.70%in HPV positive and 6.52%in HPVnegative samples and the results were significantly different(P<0.01).(2)Cytologically,the rates ofhTERC gene amplification appeared to be as follows:NILM(5.97%).ASCUS(18.75%),LSIL(10.00%)。ASC.H(66.67%),HSIL (100.00%).There Was a marked increase of hTERC amplification in patients with HSIL and above lesions(P<0.01).(3)On histology findings。the rates of hTERC gene amplification were as follows:NILM(O%),CIN I(5.45%)。CINⅡ(50.00%),CINⅢ(77.78%)。and invasive carcinoma(l OO.00%).There was a marked increase of hTERC amplification in patients wiⅡl CIN II and above lesions(P<0.01).Conclusion There was a close correlation between amplification ofhTERC and histological as well cytological lesions which increased progressively along with the severity of cytological and histological grade.The evidence of hTERC.with or without amplification,might serve as aprognostic indicator to measure the grade of lesion.
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