郭卫东,张文义,江佳富,池海谊,王文瑞,王中元,金鹰,杨红,方立群,曹务春.中国宿主动物中Amur汉坦病毒M片段全基因序列分析[J].中华流行病学杂志,2009,30(10):1051-1054 |
中国宿主动物中Amur汉坦病毒M片段全基因序列分析 |
Complete sequence of the M segment of Amur virus in rodent from China |
收稿日期:2009-03-20 出版日期:2014-09-18 |
DOI: |
中文关键词: 汉坦病毒 Amur病毒 M片段 序列分析 |
英文关键词: Hantavirus Amur virus Msegment Sequence analysis |
基金项目:1. 国家杰出青年科学基金;2. 国家自然科学基金;3. 传染病重大专项基金 |
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中文摘要: |
目的测定中国宿主动物中Amur病毒M片段全基因序列,以了解其分子特征.方法设计特异的PCR引物,用RT-PCR法分段扩增JilinAP06株M片段全长,PCR产物经克隆后进行序列测定,然后进行系统发育分析和重组分析.结果JilinAP06株M片段全基因序列共3615个核苷酸,A+T含量为59.3%,G+C含量为40.7%,包含1个单一的开放读码框架,其最大读码框架从41~3448,由3408个核苷酸组成,共编码1135个氨基酸.序列同源分析表明,JilinAP06株与中国的人源Amur HV株同源性为96.0%~97.8%,与韩国大林姬鼠来源的Soochong HV同源性为85.6%~86.7%,与HTNV原型株76-118同源性仅为79.5%,而与其他各型病毒间的同源性均低于79.0%.氨基酸同源分析,其氨基酸序列与中国的人源株Amur HV同源性为98.1%~98.4%,与韩国大林姬鼠来源的Soochong HV同源性为96.4%~97.0%,与HTNV原型株76-118同源性为91.7%,而与其他各型病毒间的同源性均低于92.0%.系统发育分析结果显示,JilinAP06与Amur病毒构成一个相对独立的分支.结论从中国宿主动物中获得了AmurHV的M片段全基因序列. |
英文摘要: |
Objective To study the complete sequence of Msegment of Amur virus in rodents and to explore their molecular characteristics. Methods Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software. Results The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76-118. Conclusion The complete sequence on M segment of Amur virus in rodent was first time identified in this country. |
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