文章摘要
沈军,朱启镕,曾玫,俞蕙,王晓红.上海地区人偏肺病毒致儿童急性下呼吸道感染的分子流行病学研究[J].中华流行病学杂志,2010,31(4):447-450
上海地区人偏肺病毒致儿童急性下呼吸道感染的分子流行病学研究
Molecular epidemiology of human metapneumovims in children with acute lower respiratory tract infections in Shanghai
收稿日期:2009-09-28  出版日期:2014-09-12
DOI:10.3760/cma.j.issn.0254-6450.2010.04.020
中文关键词: 人偏肺病毒|急性下呼吸道感染|儿童|病原学
英文关键词: Human metapnenmovirus|Acute lower respiratory tract infection|Children|Etiology
基金项目:上海市公共卫生重点学科建设项目(08GWZX0102)
作者单位E-mail
沈军 复旦大学儿科医院感染科, 上海 201102 echoshen11@yahoo.com.cn 
朱启镕 复旦大学儿科医院感染科, 上海 201102  
曾玫 复旦大学儿科医院感染科, 上海 201102  
俞蕙 复旦大学儿科医院感染科, 上海 201102  
王晓红 复旦大学儿科医院感染科, 上海 201102  
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中文摘要:
      目的 建立可靠的TaqMan-MGB实时荧光RT-PCR方法, 检测临床样本中人偏肺病毒(hMPV)N基因, 以了解2006年10月至2008年2月期间上海地区hMPV致儿童急性下呼吸道感染(ALRTI)的临床与分子流行病学特点。方法 为了明确hMPV在儿童ALRTI中的地位, 用直接免疫荧光方法检测呼吸道合胞病毒(RSV), 腺病毒, A、B型流感病毒, 1、2和3型副流感病毒7种儿童常见呼吸道病毒抗原, 结合肺炎支原体、沙眼衣原体荧光定量PCR检测结果及细菌学培养结果进行分析。结果2006年10月至2008年2月上海地区ALRTI儿童鼻咽分泌物中hMPV的检出率为3.86%(24/622), 2006-2007年冬季和2007-2008年冬季检出率分别为6.60%(14/212)和1.11%(2/180); hMPV致儿童ALRTI以<5岁儿童多见。该期间上海地区hMPV仅见A2亚型株流行。呼吸道病毒是上海地区儿童ALRTI主要病原, 在儿童ALRTI中hMPV感染可能仅次于RSV, 是常见的呼吸道病毒。结论 hMPV是上海地区儿童ALRTI的重要病原体。
英文摘要:
      Objective To understand the clinical and moleculare pidemiologic features of human metapnenmovirus (hMPV) in children with acute lower respiratory tract infection (ALRTI) from Oct. 2006 to Feb. 2008 in Shanghai. Methods We established a reliable and stable diagnostic method of TaqMan. MGB real-time reverse transcription polymeruse chain reaction (RT-PCR) to detect hMPV in the clinically manifested respiratory samples. To understand the role of hMPV in children's ALRTI, we tested other seven common respiratory viruses, using direct imlnunofluorescence assays (DFA). Results Data was analysed on the rates of detection among patients with respiratory bacteria, mycoplasma pneumonia and chlamydia among the targeted samples and enrolled patients to disphy the spectrum of respiratory pathogens and the changing pattern of pathogens with time. Of 622 samples, hMPV was detected to be positive in 24 (3.86%) samples. However, the prevalence of hMPV in the winter season of 2006 to 2007 was higher than that in the winter season of 2007 to 2008. The majority of children infected with hMPV were younger than 5 years old. Data from phyiogenetic tree analysis further demonstrated A2 genotype hMPV circulating during the study period. Conclusion Respiratory viruses were important respiratory pathogens among children with ALRTI and respiratory syncytial virus. They were the two most common virus which might be followed by hMPV that causing the diseasos. We confirmed that hMPV as an important pathogen of ALRTI in the children from Shanghai.
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