| 刘晓琳,汪照国,杨婷婷,弋英.青岛地区2007--2009年人肠道病毒71型的分子流行病学研究[J].中华流行病学杂志,2011,32(4):382-384 |
| 青岛地区2007--2009年人肠道病毒71型的分子流行病学研究 |
| Molecular epidemiology of human enterovirus 71 strains in Qingdao region-Shandong province.2007—2009 |
| 收稿日期:2010-12-07 出版日期:2014-09-10 |
| DOI： |
| 中文关键词: 手足口病 人肠道病毒7l型 VPI基凶 分子流行病学 |
| 英文关键词: nd-foot-mouth disease Enterovirus 7l VPl gene Molecular epidemiology |
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| 中文摘要: |
| 目的了解2007—2009年青岛地区引起手足口病的人肠道病毒71(EV71)型VPI基因编码区(CDS)分子流行病学特征。方法采用荧光定量PCR对咽拭子标本进行总肠道病毒(EV)、柯萨奇病毒A16(CAl6)和EV71检测。选取部分EV71检测阳性的标本进行VPl基阂CDS序列的扩增及测序，对所获得的序列进行进化树分析。结果青岛市2007—2009年的51株EV71病毒核苷酸和氨基酸的差异性分别为5．3％和1．7％。所有病毒均属于C4亚型中的C4a簇。2008和2009年的基因进化树均有多个分支产生，但每年都有一个主要分支(即优势株)。2008年的优势株与2007年山东省临沂市优势株高度同源，2008年的次优势株和2009年的优势株同与2008年安徽省阜阳市优势株高度同源。2007年的2株EV71与2007年山东省临沂市流行株高度同源。结论青岛市2008和2009年的EV71均有多个传播链存在，2008年的主传播链至2009年变为次传播链，但2008年的次传播链至2009年发展为主传播链。 |
| 英文摘要: |
| Objective To study the molecular epidemiology of human enterovirus 71(EV7 1)isolated from patients with hand．foot—mouth disease(HFMD)in Qingdao region between
2007 and 2009．Methods Throat swabs of HFMD were detected for total enterovirus(EV)。EV7 I and CAl6 bv fluorescence reverse transcription—polymerase chain reaction(ImPCR)．T11e VPl region was amplified from positive EV71 specimens and 5l nucleotide sequences of VPI gene were
SUCCESSfully sequenced．EV7 l genotypes were characterized by phylogenetic analysis．Results 5l Oingdao EV7 l strains identified between 2007 and 2009 belonged to C4a cluster of subgenotype CA and 5．3％nucleotide divergence and 1．7％amino acid divergence were found among them．The strains in 2008 and 2009 were respectively divided into severallineages on phylogenetic flees but there was a main lineage(namely prominent strain)every year．The Qingdao prominent strains identified in 2008 had high identities with the Linyi prominent strains in 2007．The Qingdao prominent strains in 2009 and the secondary prominent strains in 2008 had high identities with the Fuyang prominent strains in 2008．Two Qingdao strains in 2007 had high identities with the Linyi popular strains in 2007．Conclusion Data showed that severaI EV7l transmission chains were co-circulating in Qingdao between 2008 and 2009．The main transmission chain in 2008 became the minor one but the minortransmission chain in 2008 was transferred into the main transmission chain in 2009． |
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