| 王金桃,霍晓旭,丁玲,郝敏,樊金荣,张丽,赵维敏.叶酸与DNA甲基转移酶1在宫颈癌及癌前病变中的作用[J].中华流行病学杂志,2011,32(6):617-621 |
| 叶酸与DNA甲基转移酶1在宫颈癌及癌前病变中的作用 |
| Effect of folic acid and DNA methyltransferase 1 on cervical cancer and its precancerous lesion |
| 收稿日期:2010-12-09 出版日期:2014-09-10 |
| DOI： |
| 中文关键词: 宫颈肿瘤 癌前病变 叶酸 DNA甲基转移酶1 |
| 英文关键词: Cervical neoplasms Cervix precancerous lesion Folic acid DNA methyltransferase 1 |
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| 中文摘要: |
| 目的 探讨叶酸和DNA甲基转移酶1(DNMT1)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者100例、宫颈上皮内瘤样变(CrN)患者101例和宫颈炎(CI)患者109例为研究对象.在收集全部对象人口学特征、生殖因素等相关资料的同时,检测血清叶酸水平(RIA法)、人乳头瘤病毒16的感染状况(PCR法)和DNMT1蛋白的表达量(Westernblot法).采用SPSS 16.0分析软件,进行相关资料的t检验、x2检验、因素与疾病之间关联强度指标(OR值95%CI)的计算及交互作用分析.结果 在SCC、CIN和CI组,血清叶酸含量分别为(2.60±1.61)、(3.14±2.08)和(3.32±1.74)ng/ml,DNMT1蛋白表达水平分别为2.40±0.99、1.88±0.33和0.89±0.29,随着宫颈病变的加重叶酸含量逐渐降低,DNMT1蛋白表达水平逐渐增高,两者呈负相关关系(r=-0 186,P=0001).交互作用分析显示,叶酸水平低和DNMTl蛋白高表达在CIN和SCC组均存在正相加交互作用,OR值分别为2.50(95%CI:1.21～9.22)和6.03(95%CI:2.79～21.72),交互作用指标超额危险度、交互作用归因危险比和交互作用指数在CIN组分别为0.92、0.36和2.59,在SCC组分别为2.47、0.41和1.96.结论 血清叶酸水平低及DNMT1蛋白高表达均是导致宫颈癌和癌前病变发生的危险因素,两者存在协同作用. |
| 英文摘要: |
| Objective To explore the effect of folic acid and DNA methyltransferase 1 (DNMT1) on cervical cancer and cervix precancerous lesion. Methods 100 patients with cervix squamouscell carcinoma (SCC), 101 patients with cervical intraepithelial neoplasm (CIN) and 109 patients with cervix inflammation (CI) diagnosed by histology were included in this study. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and Western blot were used to detect the levels of serum folate, HPV16 infection and the expression of DNMT1 protein,respectively. Results The average levels of serum folate were (2.60 ± 1.61) ng/ml, (3.14 + 2.08) ng/ml and (3.32+1.74) ng/ml,and the expression of DNMT1 protein were 2.40 + 0.99,1.88 + 0.33 and 0.89 ± 0.29 in the group of SCC, CIN and CI, respectively.The relationship of folate levels and DNMT1 protein expression showed inverse correlation (r=-0.186, P=0.00l). The results in our study indicated that there was an additive interaction between low-level of serum folate and high-expressionof DNMT1 protein related to the risk of CIN and SCC, with OR value as 2.50(95%C/: 1.21-9.22) and 6.03 (95%C/: 2.79-21.72) respectively. The relative excessrisk of interaction (RERI), attributableproportion of interaction (API) and synergy index (S) were 0.92, 0.36 and 2.59 in the CIN group while 2.47, 0.41 and 1.96 in the SCC group. Conclusion The low level of serum folate and high expression of DNMT1 protein seemed to be associated with high risk of cervical cancer and its precancerous lesion. It suggested that there might be a synergistic action between serum folate and DNMT1 in the progression of cervix carcinogenesis. |
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