王慧珠,焦炳欣,田敬华,李敏,郭杰,刘颖,李兴旺,王玉光.北京、河南、新疆地区HIV/AIDS相关慢性腹泻患者隐孢子虫感染检测分析[J].中华流行病学杂志,2011,32(9):927-929 |
北京、河南、新疆地区HIV/AIDS相关慢性腹泻患者隐孢子虫感染检测分析 |
Detection of Cryptosporidium infection among HIV/AIDS patients with chronic diarrhea in Beijing, Henan and Xinjiang of China |
收稿日期:2011-07-07 出版日期:2014-09-11 |
DOI: |
中文关键词: HIV感染者和艾滋病患者 隐孢子虫 慢性腹泻 |
英文关键词: HIV/AIDS Cryptosporidium Chronic diarrhea |
基金项目:国家科技重大专项(2008ZX10005-003,2009ZX10005 -014) |
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中文摘要: |
目的 了解北京、河南、新疆地区HIV感染者和艾滋病患者(HIV/AIDS)相关慢性腹泻患者中隐孢子虫感染状况。方法收集3个地区HIV/AIDS相关慢性腹泻患者粪便,采用甲醛-乙酸乙酯沉淀法集卵,用改良抗酸染色法染色检测隐孢子虫卵囊。同时检测患者血液CD4+T淋巴细胞计数。结果253例HIV/AIDS相关慢性腹泻患者的粪便标本中有32例为隐孢子虫阳性,感染率为12.6%。北京、河南、新疆地区艾滋病慢性腹泻患者隐孢子虫感染率分别为5.97%(4/67)、16.1%(24/149)和10.8%(4/37),地区间差异有统计学意义(x2=4.431,P<0.05)。男性和女性艾滋病慢性腹泻患者隐孢子虫感染率分别为11.2%( 16/1 43)和14.5%(16/110),差异无统计学意义(x2=0.634,P>0.05);城市和农村艾滋病慢性腹泻患者隐孢子虫感染率分别为6.5%(7/104)和16.8%(25/149),差异有统计学意义(x2=5.596,P<0.05);夏秋季与其他季节的艾滋病慢性腹泻患者隐孢子虫感染率分别为14.0%(30/214)和5.1%(2/39),差异无统计学意义(P>0.05);艾滋病慢性腹泻患者处于HIV感染无症状期、有症状期、艾滋病期隐孢子虫感染率分别为0%(0/7)、21.3%(19/89)、8.3%( 13/157),差异有统计学意义(x2=9.822,P<0.01);不同CD4+T淋巴细胞计数水平(cell/μl)艾滋病慢性腹泻患者隐孢子虫感染率有差异,<200为20.4% (20/98),200~499为9.23%(12/130),≥500为0%( 0/25),差异有统计学意义(x2=10.33,P<0.01)。艾滋病中晚期慢性腹泻患者,尤其是CD4+T淋巴细胞计数<200 cell/μl的患者其感染率明显增加。结论3个地区艾滋病慢性腹泻患者隐孢子虫感染率与性别无关;患者来自农村地区、CD4+T淋巴细胞计数水平降低、疾病为中晚期患者易发生隐孢子虫感染。 |
英文摘要: |
Objective To investigate the Cryptosporidium infection and its epidemiological characteristics in HIV/AIDS patients with chronic diarrhea.Methods Stool samples collected from HIV/AIDS confirmed patients with chronic diarrhea who lived in Beijing, Henan and Xinjiang.Samples were concentrated by Formalin-Ethyl Acetate Sedimentation technique and stained by modified acid-fast stain (AFS) for the identification of oocysts by microscopy. CD4+T cells count was performed by Flow Cytometry.Results The overall infection rate of Cryptosporidium in AIDS patients was 12.6%(32/253). The infection rates of oocysts in the area of Beijing, Henan and Xinjiangwere 5.97% ( 4/67 ), 16.1% (24/149 ) and 1 0.8% (4/37) respectively. The infection rate of oocysts in the urban areas was 6.5%(7/104) while in the countryside it was 16.8%(25/149) and the difference was significantly different. However, there were no any differences discovered between the infection rates on patient' s gender or on infection occurred in different seasons. The infectious rates of ooeyst in patients on different stages of the disease were also significantly different (P<0.01).Conclusion AIDS patients infected by Cryptosporidium were not rarely seen in northern China. The rate of infection was not associated with patient' s gender but was associated with patient' s living environments. Patients living in the countryside, with lower lever of CD4+T cells counts and at the middle/late stage of the disease, Cryptosporidium infection appeared to be high. |
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