| 赵广录,于微,张娟娟,陈琳,冯铁建,王峰,洪福昌,王晓辉,李青.深圳地区1992--2008年HIV-1分子流行病学研究[J].中华流行病学杂志,2012,33(1):82-87 |
| 深圳地区1992--2008年HIV-1分子流行病学研究 |
| Study on the molecular-epidemiological characteristics of HIV-I in Shenzhen,1992—2008 |
| 收稿日期:2011-06-17 出版日期:2014-09-10 |
| DOI： |
| 中文关键词: 艾滋病病毒一1 分子流行病学 |
| 英文关键词: Human immunodeficiency virus—l Molecular epidemiolog |
| 基金项目: |
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| 摘要点击次数: 2297 |
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| 中文摘要: |
| 目的 了解1992-2008年深圳地区HIV-1亚型的流行特点。方法 抽取1992-2008年深圳地区HIV确认阳性患者血清489份，应用巢式PCR扩增膜蛋白基因(env基因)，并对PCR产物进行序列测定和分析。结果 共有464份样本获得分型结果，主要为CRF01 AE亚型(64.4％,299/464)，其次是CRF BC( 17.5％,81/464)、B'(14.7％,68/464)和B亚型(2.4％,11/464)，还发现C(0.4％,2/464)、A1 (0.2％,1/464)、CRF02_AG(0.2％,1/464)和CRF06_cpx (0.2％,1/464)亚型/重组亚型。CRF01 AE和CRF BC主要流行于异性性传播、同性性传播和静脉吸毒人群，B'亚型主要经异性、同性性传播和血液传播。另外经系统进化分析发现，不同时间段的样本出现明显的时间汇聚现象，并伴随传播途径的聚集以及交叉感染现象；各亚型/重组株样本组内及与标准参考株间的基因离散率随着时间的增加有明显增大趋势。结论 CRF01 AE重组株是深圳地区HⅣ-1的主要流行株，CRF_BC、B、B'、C、A1和CRF02_AG、CRF06_cpx亚型/重组亚型也在深圳地区少量流行,各亚型在流行过程中发生较大变异。 |
| 英文摘要: |
| Objective To investigate the epidemiological characteristics of HW-1 subtype in Shenzhen from 1992 to 2008.Methods 489 HIV-1 positive plasma samples were collected from 1992 to 2008 in Shenzhen.HIV-1 env genes were amplified by nested-PCR from RNA.Phylogenetic analysis was performed on data regarding the nucleotide sequence.Results A total of 464 sequences were amplified and genotyped.Data from this study revealed that CRF01_AE was a predominant HIV-1 subtype in Shenzhen (64.4％,299/464),followed by subtypes CRF_BC( 17.5％,81/464),B'( 14.7％,68/464) and B (2.4％,11/464).Subtype C (0.4％,2/464),A1 (0.2％,1/464),CRF02_AG(0.2％,1/464) and CRF06_cpx (0.2％,1/464) were also prevalent in Shenzhen.CRF01_AE and CRF_BC were predominant among heterosexuals,homosexuals and injection drug users,while B'was predominant among blood donors.Results from phylogenetic tree analysis showed that some of the HIV-1 clusters had been defined in CRF01_AE strains at different time or groups with different transmission routes.Cross-infections were also seen.Conclusion CRF01_AE was the predominant HIV-1 subtype in Shenzhen while CRF_BC,B,B',C,A1,CRF02_AG and a small amount of CRF06_cpx or recombinant subtypes were prevalent in this city.Different subtypes showed great variation in the process of epidemics. |
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