文章摘要
王峰,胡思玉,桂静,崔运勇,刘小立,李庆阁.PCR熔解曲线法筛查结核分枝杆菌链霉素和乙胺丁醇耐药性[J].中华流行病学杂志,2012,33(5):525-528
PCR熔解曲线法筛查结核分枝杆菌链霉素和乙胺丁醇耐药性
A rapid screening program on the resistance to streptomycin and ethambutol in Mycobacterium tuberculosis isolates by PC Rmelting curve analysis
收稿日期:2011-10-09  出版日期:2014-11-03
DOI:
中文关键词: 结核分枝杆菌;耐药性;链霉素;乙胺丁醇
英文关键词: ycobacterium tuberculosis;Drug resistance;Streptomycin;Ethambutol
基金项目:国家“十一五”科技重大专项(2008ZXl0003一004)
作者单位E-mail
王峰 深圳市慢性病防治中心病原检验科 518020  
胡思玉 厦门大学生命科学学院分子诊断教育都工程研究中心  
桂静 深圳市慢性病防治中心病原检验科 518020  
崔运勇 深圳市慢性病防治中心病原检验科 518020  
刘小立 深圳市慢性病防治中心病原检验科 518020 liuxl36@126.Com 
李庆阁 厦门大学生命科学学院分子诊断教育都工程研究中心 qgli@xmu.edu.cn 
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中文摘要:
      目的利用PCR熔解曲线法快速筛查结核分枝杆菌(MTl3)对链霉素、乙胺丁醇耐药性,并与药敏试验结果比较,评价其应用价值。方法收集深圳市2007--2009年331株MTB临床分离株,应用PCR熔解曲线法检测embB基因306、378—380、406和497、rpsL基因43、88及m基因513—517、905—908位点耐药突变,并与药敏试验结果比较。结果以药敏试验结果为标准,PCR熔解曲线法检测MTB链霉素耐药突变的敏感性为78.6%、特异性为90.1%、准确性为86.7%;检测MTB乙胺丁醇耐药突变的敏感性为83.o%、特异性为93.3%、准确性为91.8%。PCR熔解曲线法与药敏试验检测结果具有很好的一致性。结论PCR熔解曲线法能够快速、特异地检测MTB对链霉素、乙胺丁醇耐药突变,可用于临床筛查。
英文摘要:
      Objective To evaluate the effects of PCR melting curve analysis assay on arapid screening program regarding the resistance of Mycobacterium tuberculosis(MTB)clinical isolates to streptomycin and ethambutol.MethodsA total of 331 clinical isolates of MTB had been collected since 2007—2009 in Shenzhan.Mutations at codon 306,378—380,406and 497 of embB gene,codon 43,88 ofrpsl gene,and 513-517,905-908 region of rrs gene were detected by PCR melting curve analysis.Result were compared with that of conventional drug susceptibility test.ResultsCompared to drug susceptibility test,sensitivity, specificity and accuracy for streptomycin resistance were 78.6%,90.1%and86.7%,respectively while83.O%,93.3%and91.8%,respectively for etharnbutol resistance detected by PCR melting curve analysis.PCR melting curve method was in good agreement with drug susceptibility test.Conclusion PCR melting curve analysis on genetic regions associated with resistance to streptomycin and ethambutol seemed to be arapid,specific and closed-tube method so it could be used for detection of treptomycin and ethambutol resistance in MTB.
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