文章摘要
刘东瀛,刘婧,李金林,陈文,罗凡,李睛,杨占秋.湖北省武汉地区啮齿动物汉坦病毒S基因的特征分析[J].中华流行病学杂志,2012,33(8):828-831
湖北省武汉地区啮齿动物汉坦病毒S基因的特征分析
enetic analysis on S segment of bantaviruses in rodent hosts from Wuhan area, Hubei province
收稿日期:2012-02-16  出版日期:2014-09-03
DOI:
中文关键词: 汉坦病毒  啮齿动物  基因  系统发生分析
英文关键词: Hantavirus  Rodent  Gerie  Phylogenetic analysis
基金项目:周家高技术研究发展计划(863计划)(2007AA022465);国家自然科学基金(30770096)
作者单位E-mail
刘东瀛 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
刘婧 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
李金林 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
陈文 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
罗凡 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
李睛 武汉大学医学病毒学研究所/病毒学国家重点实验室 430071  
杨占秋 医学微生物学教研室 yangzhanqiu@163.com 
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中文摘要:
      目的 了解湖北省武汉地区啮齿动物自然感染汉坦病毒(HV)情况以及流行的基因型和亚型。方法 2000-2003年、2009-2011年秋冬季在武汉地区新洲、江夏区野外及居民区采用夹夜法捕鼠。对捕获的动物进行分类鉴定并取肺脏用间接免疫荧光法检测病毒抗原。抗原阳性的样本,采用RT-PCR方法扩增部分S片段核苷酸序列,构建系统发生树并进行基因分型。结果 2000-2003年捕获啮齿类动物437只,HV抗原阳性鼠肺标本24份,病毒携带率为5.49%。2009-2011年捕获啮齿类动物173只,HV抗原阳性鼠肺标本7份,病毒携带率为4.05%。褐家鼠为当地的优势鼠种。22份标本成功地用汉滩病毒(HTNV)、汉城病毒(SEOV)特异引物扩增部分S基因片段并测序。17只(13只褐家鼠,4只黑线姬鼠)鼠肺标本中扩增出SEOV部分S基因片段(nt 588~1147),分别属于第3亚型和2个新的基因亚型。5只黑线姬鼠的鼠肺标本中扩增出HTNV部分S基因片段(nt615-1141),分别属于第7亚型和1个新的亚型。结论 武汉地区流行的HV为SEOV和HTNV,并发现新的基因亚型,SEOV可能“溢出”感染黑线姬鼠。
英文摘要:
      Objective To investigate the infection and genotype of hantaviruses in rodents from Wuhan area,Hubei province.Methods Rodents were trapped in fields and residential areas of Xinzhou and Jiangxia districts of Wuhan in autumn and winter seasons,from 2000 to 2003 and from 2009 to 2011.Trapped rodents were identified,and hantavirus antigens were detected in the lung tissues with indirect immunofluorescence assay(IFA).Partial S segment sequences were amplified with RT-PCR in hantavirus antigen positive samples and then sequenced.Phylogenetic tree was constructed to analyze the genetic characteristics of hantaviruses.Results From 2000 to 2003,437 rodents were trapped,with 24(5.49%) lung tissues showed hantavirus antigen positive.From 2009 to 2011,173 rodents were trapped and 7(4.05%) were hantavirus antigen positive.Rattus norvegicus were the dominant species of rodents.Partial S segment sequences were amplified from 22 samples with Hantaan and Seoul viruses specific primers and sequenced.Partial S segments of Seoul viruses(nucleotide 588-1147) were amplified from 17 rodents(13 R.norvegicus and 4 Apodemus agrarius).Seven of these sequences belonged to 3 genetic lineage,while two novel genetic lineages were formed by 9 and 1 sequences,respectively.Partial S segments of Hantaan viruses(nucleotide 615-1141) were amplified from 5 A.agrarius.One of these sequences belonged to 7 genetic lineages,and 4 sequences formed one novel genetic subtype.Conclusion Hantaan and Seoul viruses co-circulated in Wuhan area.Hubei province.Novel genetic lineages were identified in this study and Seoul virus might have caused spillover infection in A.agrarius.
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