文章摘要
杨绍敏,李惠琴,陈立力,李林,刘永健,钟敏,李健健,杨壁珲,高丽,樊移山,李敬云.云南省2008-2009年HIV-1病毒株亚型分布[J].中华流行病学杂志,2012,33(8):836-840
云南省2008-2009年HIV-1病毒株亚型分布
HIV-1 subtype and the distribution In Yunnan province
收稿日期:2012-02-15  出版日期:2014-09-03
DOI:
中文关键词: 艾滋病毒1型  基因型  分子流行病学
英文关键词: umanimmunodeficiencyvirustype 1  Genetic subtype  Molecularepidemiology
基金项目:“十一五”国家科技重大专项(2008ZXl0001--004)
作者单位E-mail
杨绍敏 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
李惠琴 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301 787923852@qq.com 
陈立力 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
李林 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
刘永健 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
钟敏 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
李健健 云南省传染病专科医院艾滋病关爱中HIV实验室, 昆明 650301  
杨壁珲 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室  
高丽 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室  
樊移山 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室  
李敬云 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室 lijy@nic.bmi.an.cn 
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中文摘要:
      目的 了解云南省目前HIV-1流行株的基因型及其地区和人群分布。方法 收集2008-2009年云南省15个地市788例HIV感染者/艾滋病患者(HIV/AIDS)的血浆标本和背景信息,采用RT-PCR法分别扩增HIV-1 gag、pol全长基因(1584 bp和3147 bp)及env基因的C2V3片段(558 bp),序列编辑后用Genotyping及Mega 5.03软件工具确定病毒基因型,分析HIV-1株的地区和人群分布特征。结果 788例标本获得1728条HIV-1基因序列,其中全长gag基因序列599条、全长pol基因序列564条、env基因C2V3区序列525条,确定617例基因亚型,构成比分别为CRF08 BC(50.2%)、CRF01 AE(25.0%)、未知重组(10.2%)、CRF07_BC(9.2%)、C亚型(2.9%)和B(B')亚型(2.4%)。HIV-1株在云南省具有地域分布特征,可分为以临沧和昆明为代表的CRF08_BC为主的地区,以德宏和西双版纳为代表的CRF08_BC与CRF01 _AE构成比相近的地区;未知重组型在云南省少数民族中所占比例(17.0%)显著高于汉族(6.7%);异性性传播感染者和静脉注射吸毒感染者的CRF08 BC亚型均占总数的50.0%以上,但前者CRF01_AE的构成比占约30.0%,后者未知重组型和CRF07 BC的比例分别达到约15。0%。未知重组病毒株呈现两种重组模式,分别为B(B')/C重组和以CRF01 AE为母株嵌入B(B')和/或C片段重组,并以前者为主(74.6%)。结论 云南省HIV-1株组成复杂,具有显著的地域、民族和传播途径相关的特征,并出现新型重组病毒株,应密切监测。
英文摘要:
      Objective To understanding the genetic subtype and its population and regional distribution of HIV-1 strains circulating in Yunnan province.Methods 788 plasma specimens collected in 2008-2009 from 15 distracts of Yunnan,were enrolled.Viral RNA were extracted and subjected to RT-PCR.1584 bp full length gag gene,3147 bp full length pol gene and 558 bp env(C2V3) fragment were amplified and directly sequenced.Full length gag and pol genes were connected together as a complete genetic region(location on HXB2:790-5096) for genotyping.Results Of the 788 plasma specimens,a total number of 1728 genomic sequences including 599 gag,564 pol and 525 env(C2V3) were successfully amplified and sequenced,with genotype of 617 samples identified.The subtypes of HIV-1 strains circulated in Yunnan were with the order of constituent ratio CRF08_BC(50.2%),CRF01_AE(25.0%),unknown recombinant forms(10.2%),CRF07_BC(9.2%),subtype C(2.9%) and subtype B(B')(2.4%).The distributions of subtypes showed significant regional differences and could be roughly divided into two forms:the CRF08_BC predominant areas represented by Lincang and Kunming,and the areas with CRF08_BC together with CRF01_AE coexistence,represented by Dehong and Xishuangbanna.The unknown recombinant forms accounted for more HIV infection in ethnic minorities(17.0%) than in ethnic Han(6.7%,P<0.01).The distribution of subtypes varied significantly in the two primary routes of transmission for those infected through heterosexual contact.CRF08_BC and CRF0 1_AE were the dominant subtypes,accounting for 52.7% and 29.1% respectively.However,in IDUs,CRF08_BC strains accounted for half of the infection,while only 4.5% of the infections were caused by CRF01_AE,CRF07_BC while the unique recombinant forms were responsible for 15.5% infections.Of the 63 unknown recombinant forms,most(74.6%) were B(B') recombinant with C,while 25% were mosaic B and/or C fragments on the bases of CRF01_AE genome.Conclusion The subtypes of HIV-1 strains circulated in Yunnan were complicated under the significant differences of regions,ethnics or routes of transmission.
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