| 陈敏,姚仕堂,马艳玲,何翔,王继宝,程春林,杨锦,苏莹珍,李艳玲,段松,陈会超,付丽茹,贾曼红,陆林.云南省德宏州2011年HIV-1不同亚型人群分布特征分析[J].中华流行病学杂志,2012,33(9):883-887 |
| 云南省德宏州2011年HIV-1不同亚型人群分布特征分析 |
| Distribution of HIV-1 subtypes among differen populations in Dehong prefecture, Yunnan province, in 2011 |
| 收稿日期:2012-03-01 出版日期:2014-09-17 |
| DOI：10.3760/cma.j.issn.0254-6450.2012.09.002 |
| 中文关键词: 人类免疫缺陷病毒一l 亚型 分布特征 |
| 英文关键词: HIV-1 Subtype Distribution characteristics |
| 基金项目:国家“十二五”科技重大专项(2011ZXl0004-903) |
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| 中文摘要: |
| 目的 研究云南省德宏州HIV-1亚型的流行特点。方法 收集2011年1-5月德宏州HIV-l抗体确认阳性血浆样品300份，提取RNA后用巢式PCR对gag基因和env基因的部分片段进行扩增。扩增产物经基因序列测定，所得序列通过构建系统进化树确定HIV-1的分子亚型。结果 结合2个基因片段，共有222份样品获得分型结果，主要为C亚型(43.2%, 96/222)，其他依次为独特重组形式(URF; 27.O%, 60/222)、CRF01_AE(21.2%, 47/222)、CRF08_BC(5.0%, 11/222)、B’(2.3%, 5/222)和CRF07_BC(1.4%, 3/222)。C亚型是性传播人群和注射吸毒人群中的首要亚型，但不同传播途径人群中亚型分布具有不同的模式。异性传播人群包括该地区检测到6种基因型，主要为C亚型(40.7%,70/172)、CRFOI_AE(25.O%, 43/172)和多种URF(25.0%, 43/172)，呈现亚型分布的多样化。注射吸毒人群中包括除B'和CRF07_BC以外的3种亚型及多种URF，主要为C亚型(54.8%, 23/42 J和URF(38.1%, 16/42)，呈现亚型相对集中的趋势。URF比例的显著上升成为该地区的另一个流行特点，包括BC重组(41.7%, 25/60)和与CRF01 _AE相关的重组(58.3%, 35/60)，这两种URF在性传播和注射吸毒人群中分布的差异无统计学意义。结论 德宏州HIV-1流行株较为复杂，存在5种亚型和多种URF．其中C亚型为主要流行株，不同传播途径亚型分布模式存在差异． |
| 英文摘要: |
| Objective To investigale the dislribution of HIV-l subtypes in Dehong prefecture．Yunnan province, In 2011. Methods 300 HIV-l positive plasma samples were collected from Jan 2011 to May 2011 in Dehong prefecture. HIV-l gag genes and env genes were amplified by nested-polynlerase chain reaction (PCR) from viral RNA．After sequencing, the HIV-1 subtypes were determined by phylogenetic analysis. Results Based on the phylogenetic trees of gag gene and env gene fragments, a total of 222 samples were genotyped. Subtype C was the predominant strain in Dehong(43.2%, 96/222), followed by unique recombinant forms (URFs, 27.O%, 60/222), CRF0l_AE(21.2%, 47/222), CRF08_BC(5.0%, 11/222), B'(2.3%, 5/222) and CRF07_BC(1.4%, 3/222). Subtype C strains were predominant in both heterosexually transmitted population and intravenous drug users(IDUs), but difierent subtype distribution patterns were found in these two populations. All 6 genotypes including subtype C(40.7%, 70/172)．CRF0l_AE(25.O%, 43/172), and URFs (25.0%, 43/172) found in this area among heterosexually transmitted population, which showed the diversity of genotypes in this population. Except subtype B and CRF07_BC．the other 3 subtypes and URFs were detected among IDUs, mainly including subtype C(54.8%, 23/42) and URFs(38.1%, 16/42), which showed the concentration trend of genotypes distribution among IDUs . The proportion of URFs increased significantly in this area, including the new BC recombinants(41.7%, 25/60) and CRF01_AE relative URFs(58.3%, 35/60)However，the distributions of these two URFs among heterosexually transmitted population and IDUs showed no statistical significance. Conclusion The distribution of HIV-1 strains prevailing in Dehong prefecture was diversity, including 5 subtypes and a varlety of URFs, of which subtype C was the predominant strain. The distribution patterns of subtypc were different among different populations. |
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