| 李洁,曲梅,贾蕾,张新,刘白薇,黄芳,黎新宇,王全意.北京市2010年手足口重症病例EV71检出情况及病原学分析[J].中华流行病学杂志,2012,33(9):926-929 |
| 北京市2010年手足口重症病例EV71检出情况及病原学分析 |
| Etiological detection of severe hand-food-mouth disease and related genetic characteristics of enterovirus type 71 infectionin Beijing, 2010 |
| 收稿日期:2012-08-16 出版日期:2014-09-17 |
| DOI：10.3760/cma.j.issn.0254-6450.2012.09.011 |
| 中文关键词: 手足口病 肠道病毒71型 序列分析 |
| 英文关键词: Hand-food-mouth disease Enterovirus type 71 Sequence analysis |
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| 中文摘要: |
| 目的 了解2010年北京市手足口重症病例肠道病毒(EV)检出情况及EV71病原学特征。方法 荧光定量RT-PCR检测EV7I和柯萨奇病毒A组16型(CoxA16)。采用RD细胞对检测阳性的咽拭子进行病毒分离培养和鉴定，对EV71分离株的VPI基因序列进行种系发生分析。结果 2010年北京市手足口重症病例442例荧光定量RT-PCR检测阳性253例，阳性检出率为57.24%，其中EV71为54.55%(138/253)，CoxAl6为5.93%(15/253)，其他肠道病毒为39.53%(100/253)。VPl基幽序列分析表明2010年北京市手足口重症病例的12株EV71分离株均属于C4a基因型，核苷酸同源性为97.2%～100.0%；与2007—2010年北京EV71分离株、2007年山东手足口重症病例、2008年安徽阜阳及2008年山东手足口重症及死亡病例EV71分离株VPl基因序列核苷酸同源性为94.0%一99.9%。结论 北京市2010年手足口重症病例由C4a基因型EV71引起，其巾至少有4个病毒链在重症病例发病中起作用。分离的12株EV71与安徽阜阳地区2008年手足口重症病例分离株亲缘关系较近。 |
| 英文摘要: |
| Objective To study the etiological detection on samples from severe hand-foot-mouth disease (HFMD) cases and the genetic characteristics of enterovirus type7l(EV71) isolates from severe patients in Beijing, 20lO. Methods Real-time RT-PCR was used to detect EV71 and Coxsackievirus A16 (CoxAl6) and RD cells were used to separate virus strains from samples. Homogeneity of EV71 isolated strains were also analyzed. Results Four hundred and fourty-two severe cases were detected and 253 were positive, taking up 57.24% of the total(253/442). The overall positive detection rate on EV71 was 54.55%(138/253), with CoxAl6 as 5.93% (15/253), and with other enterovirus group was 39.53%(100/253). The nucleotide homogeneity of VP1 within these 12 strains was 97.2%-100.0%, and with Beijing strains in 2007-2010. Shandong strains in 2007 and Anhui Fuyang strains in 2008 and the Guangdong strains in 2008 as 94.0%-99.9%. Conclusion Severe HFMD cases were most oftenly caused by EV71 but less caused by CoxAl6 or other enterovirus. The HFMD in 2010 in Beijing was mainly caused by Ev7l subgenotype C4a with 4 transmission chains. Twelve isolated EV71 strains had high homogeneity with strains isolated from severe cases in Anhui Fuyang in 2007. |
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