| 董晓莲,姚晴青,王学才,徐海涛,王晓丽,陈胜玉,唐智峰,郑英杰.母婴人群隐匿性乙型肝炎病毒感染及病毒株进化特征分析[J].中华流行病学杂志,2013,34(3):210-213 |
| 母婴人群隐匿性乙型肝炎病毒感染及病毒株进化特征分析 |
| Prevalence of occult hepatitis B virus infection and its phylogenetic features among mother—teenager pairs |
| 收稿日期:2012-08-26 出版日期:2014-09-26 |
| DOI： |
| 中文关键词: 隐匿性乙型肝炎病毒感染 显性乙型肝炎病毒感染 进化特征分析 基因型 |
| 英文关键词: Occult hepatitis B virus infection Overt hepatitis B virus Infection Phylogenetic analysis Genotype |
| 基金项目:浙江省医药卫生科学研究基金计划(A类)(2009A203)；国家科技重大专项(2008ZXl0002—012) |
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| 中文摘要: |
| 目的了解母婴匹配人群隐匿性HBV感染(OBI)及显性和隐匿性病毒株s基因的变异。方法基础调查时随访135对母婴，填写调查表及采血样，采用ELISA检测HBsAg，Real—time PcR测定病毒载量，PCR扩增HBV S基因并进行进化分析。结果共采集母血94份，其子女血样101份，OBI感染率分别为10．0％和2．0％，母亲显著高于其子女；显性和隐匿性病毒株病毒载量中位数分别为399．9 IU／ml和247．6 IU／ml，两者差异无统计学意义；OBI病毒株有l株为B基因型、adw血清型，其余7株均为C基因型、adr血清型；显性HBV感染病毒株有15株为B基因型、adw血清型，其余8株为c基因型、adr血清型；OBI病毒株中C基因型比例显著高于显性HBV病毒株。结论母婴人群存在一定比例的OBI，应重视其预防。 |
| 英文摘要: |
| Objective Prevalence of occult hepatitis B virus (HBV) infection (OBI) was investigated in a paired mother-teenager population and HBV S gene variation including overt and occult HBV,was determined.Methods A follow-up study based on an initial survey of 135 mother-teenager pairs was carried out through collection of questionnaires and blood samples HBsAg were detected by ELISA method,viral load by PCR amplification and HBV S gene by phylogenetic analysis.Results 102 pairs of subjects were followed-up.Blood samples from 94 mothers and 101children were collected.OBI prevalence in mothers was 10.0％ (6/60),significantly higher than 2.0％(2/101) in teenagers.Medians of viral load were 399.9 IU/ml and 247.6 IU/ml in overt and occult HBV strains,but without significant difference.1 occult HBV strain belonged to genotype B with serotype adw while the other 7 were genotype C with serotype adr.15 of the overt HBV strains belonged to genotype B with serotype adw and the other 8 were genotype C with serotype adr.Proportions of genotype-C strains were significantly higher in occult HBV strains than in overt HBV strains.Conclusion OBI was seen in teenage-mother population. |
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