文章摘要
吴建茹,朱一,谢彦昕,周良佳,徐金水,还锡萍,王蓓.江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析[J].中华流行病学杂志,2013,34(3):259-262
江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析
Analysis on Mycoplasma pirum infection in male HIVIAIDS patients and related 16S rRNA genes in Jiangsu province
收稿日期:2012-11-06  出版日期:2014-09-26
DOI:
中文关键词: 梨支原体;HIV感染者和艾滋病患者;pl基因;16S rRNA基因
英文关键词: Mycoplasma pirum;HIV/AIDS;p l gene;1 6S rRNA gene
基金项目:国家自然科学基金(30872156)
作者单位E-mail
吴建茹 210009 南京, 东南大学公共卫生学院流行病与卫生统计学系  
朱一 210009 南京, 东南大学公共卫生学院流行病与卫生统计学系  
谢彦昕 210009 南京, 东南大学公共卫生学院流行病与卫生统计学系  
周良佳 210009 南京, 东南大学公共卫生学院流行病与卫生统计学系  
徐金水 210009 南京, 东南大学公共卫生学院流行病与卫生统计学系  
还锡萍 江苏省疾病预防控制中心  
王蓓 江苏省疾病预防控制中心 wangbeilxb@Sell.edu.crl 
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中文摘要:
      目的 了解梨支原体(Mpi)在男性HIV感染者中的流行情况,复核鉴定部分Mpi 16S rRNA基因片段序列.方法 以江苏省男性HIV/AIDS为研究对象,采集其首段尿,用巢式PCR法检测Mpi,并描述该人群中Mpi的感染情况.纯化PCR产物进行Mpi 16S rRNA基因片段序列测定,采用Mega 4.0软件的多序列排列程序对支原体临床株和下载序列进行同源位排列、比对,利用Neighbor-Joining构建系统发育树.将Mpi的pl基因序列信息输入Vector NTI Advance 11.0软件进行分析,并推算其编码的氨基酸序列,将推断出的理论氨基酸序列与其他支原体pl全序列进行同源性分析.结果 Mpi总感染率为21.5%.18株临床分离株几乎全部汇聚为一个分支,相似度>90%,且与实验室保存Mpi标准株在同一分支,Mpi的pl蛋白与穿通支原体HF-2同源关系较近.结论 Mpi的感染率与以往调查结果相比明显增高,且临床分离株具有较高同源性.
英文摘要:
      Objective To understand the incidence rates of both typhoid fever and paratyphoid fever in the high prevalent areas of Guizhou province so as to provide evidence for the development of programs on comprehensive intervention and effectiveness evaluation.Methods Six townships in Pingba county were selected as intervention areas while six townships in Kaiyang county were taken as control.All hospitals and clinics were classified into A,B and C types according to its level and the capacity of the blood culture.Surveillance on typhoid and paratyphoid fever was conducted based on all population and all hospitals,clinics and county CDCs among the patients with unknown fever.Results In the surveillance area in those two counties,there were 12 944 blood samples from patients with unknown fever which have been tested and cultured.Among them,200 strains of Salmonella including 16 typhoid strains,184 paratyphoid A strains were identified,with the total positive rate as 1.55%.The positive rate before the intervention program was higher than the after.The detection rate was 1.91% in the type A hospitals.39 strains of Salmonella have been cultured from 2039 samples which accounting for 19.50% (39/200) of the total strains.4315 blood samples were cultured at the 'Class B' sites which isolated 82 strains of Salmonella,accounting for 41.00%(82/200),with a detection rate as 1.90%.6590 samples were cultured at the 'Class C' sites,which identified 79 strains of Salmonella,accounting for 39.50% (79/200),with a detection rate as 1.20%.The detection rate was much higher before the use of antibiotics than after using them (P<0.05).The annual peak time of positive detection was in spring and fall.The outbreaks or epidemics often appeared in the same places,with farmers,students as the high-risk populations.Symptoms of both typhoid and paratyphoid fever were not typical.Conclusion Typhoid and paratyphoid monitoring programs which covered primary health care institutions in the high incidence area seemed to be effective in reflecting the pictures as well as the burden of both typhoid and paratyphoid.
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