| 桑少伟,赵仲堂,索继江,邢玉斌,贾宁,高岩,杜明梅,谢丽君,刘伯伟.北京地区急性胃肠炎患者诺如病毒分子流行病学调查[J].中华流行病学杂志,2013,34(3):263-266 |
| 北京地区急性胃肠炎患者诺如病毒分子流行病学调查 |
| Study on the molecular epidemiological characteristics of norovirus in acute gastroenteritis of Beijing |
| 收稿日期:2012-10-09 出版日期:2014-09-26 |
| DOI： |
| 中文关键词: 诺如病毒 急性胃肠炎 分子流行病学 |
| 英文关键词: Norovirus Acute gastroenteritis Molecular epidemiology |
| 基金项目:国家自然科学基金(30872155，30972523)；国家科技重大专项(2009ZXl0004—204)；全军医学科技“十二五”科研项目(AWSllL009。11BJZ01) |
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| 中文摘要: |
| 目的 探讨北京地区急性胃肠炎患者诺如病毒分子流行病学特征.方法 采用RT-PCR扩增核酸和测序分型方法.结果 共收集腹泻标本685份,检测诺如病毒阻性66份,阳性率为9.6％.检出率在患者性别、年龄、住院和门诊间的差异均无统计学意义;诺如病毒胃肠炎患者时间分布也无明显季节性.66份阳性标本采用ORF2区测序分型,其中43份可分型.GⅠ组占22.7％,包括GⅠ.3型2份、G Ⅰ.4型1份、GⅠ.5型4份、GⅠ.7型3份;GⅡ组占77.3％,其中以GⅡ.4型为主(60.6％),其他包括GⅡ.2型1份、GⅡ.3型1份,GⅡ.5型1份、GⅡ.6型2份、GⅡ.7型1份、GⅡ.12型7份.6例出现GⅠ组和GⅡ组混合感染,其中可分型的有G Ⅰ.3/GⅡ.7、GⅠ.5/GⅡ.5、G Ⅰ.4/GⅡ.4.结论 北京地区急性胃肠炎患者诺如病毒呈现基因多样性,且以GⅡ组为主,其中GⅡ.4/2006a和2006b是主要的流行变种,存在GⅠ和GⅡ组混合感染现象. |
| 英文摘要: |
| Objective To investigate the prevalence ofMycoplasma pirum (Mpi) in male HIV infected patients,and to identify the 16S rRNA gene of Mpi.Methods The first void urine of male HIV/AIDS patients in Jiangsu province was collected for Mpi detection.Purified 16S rRNA gene PCR production was sequenced for analysis on its identification,homogeneity and phylogenetic tree.P1 protein sequence of Mpi was analyzed by Vector NTI Advance 11.0 to calculate the coded amino acid sequence.Homogeneity analysis was conducted between the theoretical amino acid sequence of Mpi and other Mycoplasmas.Results The prevalence of Mpi in male HIV/AIDS patients was 21.5％while the Mpi prevalence rates in different age groups were significantly different (x2Mpi=124.63,P＜0.01).The homogeneity of 18 strains of Mpi was higher than 90％.Conclusion The Mpi prevalence seemed much higher than the results from previous detection on HIV/AIDS patients,suggesting that more attention should be paid on AIDS treatment.More bioinformatic research on gene/nucleotide sequence analysis and forecast should be carried out to identify the molecular characteristics of Mpi. |
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