文章摘要
丁朋举,惠明,梁倩,张乾,李卫民,黄海荣.中国北方部分地区北京谱系结核分枝杆菌群体遗传关系的分析[J].中华流行病学杂志,2013,34(4):374-378
中国北方部分地区北京谱系结核分枝杆菌群体遗传关系的分析
Origin, phylogeny, and spread of Mycobacterium tuberculosis Beijing lineage in the five provinces of northern China
收稿日期:2012-09-25  出版日期:2014-09-03
DOI:
中文关键词: 结核分枝杆菌,北京谱系;系统发育;分子方差分析;基因流动;最小跨度树
英文关键词: Mycobacterium tuberculosis,Beijing lineage;Phylogeny;Analysis of molecular variance;Gene flow;Minimum spanning tree
基金项目:国家自然科学基金(81273144)
作者单位E-mail
丁朋举   
惠明   
梁倩   
张乾   
李卫民  1wm_18@hotmail.tom 
黄海荣   
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中文摘要:
      目的 <\b>应用分子遗传学方法探讨234株北京谱系结核分枝杆菌的起源进化及群体间基因流动特征.方法 <\b>对来源于中国北方五省区(黑龙汀、吉林、辽宁、内蒙古和宁夏)234株北京谱系结核分枝杆菌进行24位点可变数目串联重复序列(VNTR)基因分型,计算各VNTR位点的等位基因多态性(h);从个体水平分析其系统发育,即构建NJ树和最小生成树;构建群体水平系统发育树,并在此基础上通过贝叶斯模型计算最近共祖年代;通过分子方差分析了解五省区菌株间的基因流动情况.结果 <\b>24个VNTR位点的等位基因多态性较低(h值:0.000 ~ 0.744).234株北京谱系结核分枝杆菌分散存在NJ树各个进化分支,最小生成树中约62.0%(145/234)的菌株被划分到同一“克隆复合群”.群体水平系统发育树显示234株菌与MIRU-VNTRplus数据库(http://www.miru-vntrplus.org)中的北京谱系结核分枝杆菌的遗传关系最近(Bootstrap值为100);最近共祖年代为5308年(95%CI:4263 ~ 6470年).通过分子方差分析发现吉林与黑龙江、辽宁菌株之间,内蒙古与宁夏菌株之间遗传分化系数的差异无统计学意义(P>0.05).结论 <\b>中国北方五省区北京谱系结核分枝杆菌的遗传相似性较高,菌株间种系发生关系不明显.推测这些菌株由近期(约5000年前)结核分枝杆菌北京谱系的某一“克隆”进化而来.地理位置接近的区域,菌株间存在基因流动现象.
英文摘要:
      Objective <\b>Using methodology of molecular genetics to explore the origin,phylogen,and gene flow of Mycobacterium tuberculosis (MTB) Beijing lineage in the five provinces from northern China,including Heilongjiang,Jilin,Liaoning,Neimenggu and Ningxia.Methods <\b>234 MTB Beijing lineage strains were genotyped by 24 Variable Number Tandem Repeat (VNTR),and the h (the allelic diversity) value of each VNTR locus was calculated.On individual level of phylogeny,it was constructed Neighbor-Joining (N-J) tree and minimum spanning tree (MST).Phylogenetic tree was built at the population level,and the most recent common ancestor (TMRCA)was estimated through Bayesian model.Molecular variance (AMOVA) was used to understand the gene flow among strains discovered from the five provinces.Results <\b>Allelic diversities of the 24VNTR loci were low (h:0.000-0.744).234 strains of MTB Beijing lineage were dispersed in individual branch of the N-J tree,with 62.0% (145) of them grouped to the same "colonial complexes" in MST.At the population level,the evolution relationship of 234 strains appeared the closest to Beijing lineage,which was from MIRU-VNTRplus database,and the bootstrap was 100.The TMRCA was 5308 (95% CI:4263-6470) years.Differences of pairwise Fst values acquired by AMOVA between Jilin and Heilongjiang,Liaoning,Neimenggu and Ningxia,were not statistically significant (P>0.05).Conclusion <\b>The genetic similarity of Beijing lineage MTB from the five provinces of northern China was high.The phylogeny branches had no characteristic dispersal in each province.It was speculated that these strains showed an evolution from a clone of MTB Beijing lineage (about 5000 years ago).The gene flow was taking place between neighboring zones.
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