| 刘晔华,王世瑜,张坚磊,穆红.医院内感染耐甲氧西林金黄色葡萄球菌分型及临床分析[J].中华流行病学杂志,2014,35(1):71-76 |
| 医院内感染耐甲氧西林金黄色葡萄球菌分型及临床分析 |
| The Clumping factor B gene typing in discriminating the strains of nosocomial-acquired methicillin—resistant Staphylococcus aureus |
| 收稿日期:2013-07-28 出版日期:2014-09-02 |
| DOI： |
| 中文关键词: 耐甲氧西林金黄色葡萄球菌 凝集因子B 可变串联重复序列 基因分型 |
| 英文关键词: Methicillin—resistant Staphylococcus aureus Clumping factor B gene Variable—number tandem repeat Genotyping |
| 基金项目: |
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| 中文摘要: |
| 目的研究天津市第一中心医院内感染耐甲氧西林金黄色葡萄球菌(MRsA)的clfB分型。方法对2006—2012年天津市第一中心医院已进行SCCmec分型且MLST分型为ST239的临床非重复分离的MRSA，从ICU(n=35)和非ICU科室(凡=7)共选取42株进行clfB分型。结果42株MRSA均属于clfB的系列3，ICU的35株MRSA可分为6个型别，3.52占42.9％，3-52E占37.1％，3-50占8.6％，3-52C占5.7％，3-50A和3-50E分别占2.9％；非ICU的7株MRSA分属3个型别，3.52占42.8％，3-52E和3.50分别占28.6％；clfB分型联合SCCmec分型效果(分型指数ID=0.767)优于单纯应用clfB(ID=0.688)和SCCmec(ID=0.303)，clfB／SCCmec联合分型可以将系列3的42株MRSA分为10个型别，其中SCCmecIII—elfB3.52E是主要克隆，40.4％。相比非ICU科室，ICU感染MRSA的患者住院时间较长、使用抗菌药物治疗时间较长，差异有统计学意义(P<0.05)。结论基于可变串联重复序列的clfB分型方法分辨力高，可用于MRSA快速分型。 |
| 英文摘要: |
| 0bjective To evaluate the clfB typing method in discriminating the ST239methicillin.resistant Staphylococcus aureus(MRSA)isolated from patients under nosocomial infection in Tianjin first central hospital SO as to access the elinical risk factors and outcomes of the MRSA nosocomial infection from ICU and non.ICU departments.Methods Forty-two stains of MRSA with known SCCmec type were chosen in bom ICU(n=35)and non.ICU(几=7)wards.from 2006 to 2012，of which MLST genotype was ST239.Clinical risk factors and rates on drug resistant to MRSA were counted.respectively.Results AU the isolates of MRSA belonged to the same lineage 3 and 6 heplotypes.based on clfB variable.number random repeats typing.Thirty.five isolates from ICU belonged to 6 heplotypes，among which elfB3-52，3-52E，3-50，3-52C，3-50A and 3-50E were accouted for 42.9％，37.1％.8.6％，5.7％，2.9％and 2.9％，respectively.Seven isolates from non-ICU belonged to 3 heplotypes，in which 3-52，3-52E and 3-50 were accouted for 42.8％，28.6％，28.6％，respectively.Whell clfB typing was combined with SCCmec typing in use.results showed that the index ofdiscrimination as 0.767.beaer than clfB(ID=0.688)or SCCmec(ID=0.303)used alone.SCCmec III-clfB3—52E seemed as the major clone among the 10 heplotypes of clfB／SCCmec typing，which was accounted for 40.4％.There were significant differenees on the length of hospitalization (P<0.005)and the duration of antibiotics use(JP<0.05)between ICU and non.ICU.Conclusion The clfB typing method which Was based on vailable.numbers of tandom repeats showed powerful abilitv of resolution.It could also be combined with MLST and SCCmec typing to be used in local epidemiological investigatioas. |
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